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Questions related from G V Girish
If any scientist ihas developed a Immortal heterohybridoma line secreting monoclonal anti-D antibodies, we are keen to collaborate to take the research to clinical level for conducting the...
09 September 2018 4,685 0 View
Hi, We into developing hCG, FSH, LH biosimilar expressing CHO clones. Want to collaborate, or seek consultancy from the experts in the field. regards,
09 September 2018 4,139 0 View
Our lab is working on developing the stable gene expressing clone in DG44 cells,. After transfection, transient gene expression is working, after growing the cells in the media with out GHT and...
08 August 2018 7,264 3 View
Hi We are Looking for retroviral vector system for transducing DG44 cells with GOI to produce expressing clone. Suggestion, collaboration are welcome.
03 March 2018 6,502 1 View
what is the difference in running a E.Coli Fermentor and Mammalian CHO cell bioreactor ?
12 December 2017 5,270 0 View
Want to learn/know the 5'-ITR and 3'-ITR sequence of the Sleeping beauty transposon for designing the experiments working with Sleeping beauty transposase.
11 November 2017 3,732 3 View
Working on the DG44 CHO cells for recombinant protein expression, we have been getting the low expression of 0.5ug/ml recombinant protein and also Gal-a(1,3)Gal glycosylation of protein....
09 September 2016 8,058 1 View
Am working with cloning, and plasmid has an Sap1 restriction site at Forward and reverse (purchased the cut plasmid from an company, they call it Electracloning) and the insert gene has forward...
07 July 2016 8,239 3 View
Beta hCG expression is not seen, took of native signal peptide (20aa) of beta-hCG and replaced with human albumin signal peptide (18aa). Still B-hCG expression is not seen in the HEK293 cells. We...
04 April 2016 6,417 2 View
Want to introduce UCOE region before the hCMV promoter in the pDUO2 plasmid already cloned with gene alpha and beta at MCS1 and MCS2,how to do this.
07 July 2015 5,761 2 View
We are working with pDUO2 plasmid and after transfecting it getting low expression of protein. I want to place A2UCOE sequence [HNRPA2B1 and CBX3] before the promoter for better expression, if any...
07 July 2015 3,561 2 View
Aprotinin is a protease inhibitor and I have my sample in very dilute form. I can concentrate and use but need to know what is the least amount of aprotinin this assay can predict. BAEE is used as...
03 March 2015 7,471 1 View
I am doing affinity chromatography for Aprotinin, I am using trypsin affinity column,equilibration and binding of aprotinin ll be done with Tris HCl buffer pH 8.0 and sodium chloride concentration...
03 March 2015 1,153 5 View
Hi, We donot have instrument to quantify DNA/RNA in our lab, if any lab having an used working instrument of Amersham Hoefer DQ200 DyNA Quant 200 DNA Dynaquant Fluorometer please kindly gift us,...
01 January 1970 1,039 0 View
