It will depend on the absorbance of the inhibitor at the waveleght of work. So, If these inhibitors absorb at 234nm they will interfere in your assay and they should not be used at very high concetrations. Other parameter is their solubility in the assay medium but I assume they are fully soluble at the working concentrations.

Best of all use the concentation conditions you can find in the literature. Many LOX inhibitors have a strong UV absorbance. This depends on the exact compound. To be sure you can record the UV spectrum of diluted inhibitor solution at concentration you propose to use. The absorbance at 235 nm should be significanly less than 1 AU.

1- As Joaquin said check the OD values at the wavelength you measure your enzyme activity as a function of your inhibitor concentration.

2- Depends on your inhibitor check if it changes the pH of the solution.

3- Make sure that high concentration do not denatures your enzyme.

4- Plot enzyme activity vs inhibitor concentration. When it reaches plateau you don't need to over increase the inhibitor concentration

good luck with your experiments

Depends on what you are trying to do...

I agree with previous answers. The overall challenge is not the concentration itself, but the absorbance of the inhibitor, plus all other components of the assay, at the desired concentration. Use the Beer-Lambert equation (A=ebc) where A=absorbance, e = molar absorptivity, b= cuvette width and c= the proposed concentration, to estimate the absorbance at the desired working concentrations. If the molar absorptivity is not immediately available, it can be calculated by measuring the absorbance of a solution with a known concentration. This should give you a good idea of the feasibility of the desired inhibitor concentration.

all the answers are correct to sum up you may do two things

1. check your saturation point of your enzyme you will find it when you plot your enzyme activity vs substrate concentration when it reaches plateau there you may find the highest concentrations you can use in your assay

2. check the OD values of your inhibitors they shouldnt affect your assay so your ODs perform more than 1 value intensity...if they do you have to get the inhibitors intensity and subtract them from your observed OD values

3. be careful that your inhibitors dont denature your enzyme

Good luck

It is almost correct, sometimes we can use more high consentration for study how activity depends on inhibitor consentration.

Yes, you can test. we can test at any concentration. Inhibition depends on compound or extract potency.