Joe S Matarlo

4 Questions 19 Answers 0 Followers

Questions related from Joe S Matarlo

What is the next step after poly-alanine molecular replacement?

After a nightmare of MR trials, I've finally got a resonable match using poly-ala model by first pruning (gedit) then chainsaw. Any recommendations of a good site for the next steps would be...

07 July 2014 6,221 4 View

Does anyone know an assay for an enzyme inhibited by O2?

I am convinced that my enzyme is inhibited by atmospheric oxygen. One supporting evidence is that translation of this protein is only seen in hypoxic conditions. I want to support my hypothesis....

25 March 2014 6,647 4 View

Anyone know how to get a clean sample with SEC?

I have tried multiple buffers/conditions/parameters to get a clean sample from size exclusion chromatography SEC using sephadex 200 AKTA fPLC. Anyone have any suggestion how i get a pure protein...

17 February 2014 9,690 5 View

What is the best way to prevent membrane protein aggregation?

I'm expressing a membrane protein, and it seems to be prone to aggregating during purification. I have played with the pI-pH of buffer. What other option(s) are there?

31 January 2014 8,938 39 View