Hi, We're planning to do a ChIP-qPCR (on some genes (up/down regulated) found in previous analysis). The genes we'd liKe to choose for the CHIP-qPCR experiment should be enriched by the H3/H4 repressive marks (H3K9me3/H4K20me3). Therefore I'd be grateful if someone can show how to find the H3/H4 methylation pattern for a given gene (data-base....)
Many thanks in advance
yes, UCSC has several available data sets which you can visualize in the genome browser, by selecting the histone mark, the tissue from which the ChIP was done and the name of your gene.
Additionally, you can go to NCBI, click on GEO data set and write H3k9me3 ChIP for instance, and then you click search and you will get all public data sets available for this histone mark in which you can check your gene of interest under the condition tested there. Best wishes, Adriana
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