In your opinion, what could motivate people to wear masks and do social distancing to stop covid-19?

28 December 2020 5,149 56 View

Can we help other researchers by suggesting research topics in the field of translation studies?

One of the goals of the scientific platforms, including Research Gate, is to help researchers to update their knowledge regarding the recent developments in research in their area of study. We...

08 October 2020 9,037 24 View

Which type of translation? Texts or materials?

It is a problem when we make a search for something, theoretical or practical, related to translation "between two languages" and then we come out with the list of attractive titles and then we...

23 September 2020 1,186 8 View

Theoretical or practical translation?

21 September 2020 1,268 8 View

How to determine the optimum order of an ARIMA(p,d,q) model?

The original series is nonstationary as it has a clear increasing trend and its ACF plot gradually dampens. To make the series stationary, what optimum order of differencing (d) is needed?...

25 July 2020 6,930 4 View

What is the main difference(s) between the two-parallel linear antenna array and rectangular antenna array in a smart antenna system?

Welcome Everyone, What is the main difference(s) between the two-parallel linear antenna array and rectangular antenna array in a smart antenna system? For example, a two-parallel linear antenna...

24 June 2020 2,520 1 View

How to obtain Remote Sensing Reflectance from Surface Reflectance?

I have downloaded one EO-1 Hyperion image, removed the bad bands, de-stripped the image using THOR De-striping in ENVI and applied the atmospheric correction using FLAASH in ENVI. Now I have the...

16 June 2020 9,162 1 View

Iam looking for a laboratory space to synthesise a novel organic chromophores for dye sensitized solar cells??

Iam a Ph.D student working on synthesis of novel organic chromophores for dye sensitized solar cells, the organic chromophores to be synthesise will be based on indoline, benzothiophenes,...

21 May 2020 2,946 3 View

Can data-augmentation techniques be applied for numeric data-sets ? If so, please suggest some of the techniques?

Like there are data-augmentation techniques for image classification and text-based data, are there any analogous techniques for numeric data-sets that can be used to expand the size of a scanty...

16 March 2020 2,839 11 View

How to get boron nitride nanotubes structure from boron nitride nanopartle powder?

I prepared boron nitride nanoparticle in powder form and I want to get nanotubes structure. Please tell me any possible method?

23 February 2020 7,782 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View