What is the general protocol for isolation of probiotics from samples like milk or fecal?

More Ashvini Nagesh Angal's questions See All

Can any one describe media composition of half strength MRS /minimal media for lactobacillus?

need exact modified MRS media composition for lactobacillus / at what percent can lower the amount of meat extract and peptone or any suggested minimal media composition for lactobacillus???

11 September 2018 9,048 1 View

Can anyone share standards for synbiotic synergy evaluation for invitro analysis?

I need very preliminary and starting point experimentation for synbiotics where looking for the value addition of prebiotic for promoting growth of probiotics and metabolites.what could be the...

23 July 2018 2,030 1 View

Lactobacillus sporogenes and Bacillus coagulants is same or different?

What i understood is is, Lactobacillus sporogenes is a heat stable, gastric acid resistant strain of probiotic bacteria. It was the original name of the Bacillus Coagulans probiotic strain. and...

29 May 2018 9,511 6 View

On what basis Chitosan and TPP concentration and ratio will be fixed in formulating chitosan nanoparticles ?

I have gone through literature, but it was confusing to selecting

09 September 2015 6,237 23 View

Which are the software used for calculation of pharmakokinetic study parameters?

For auc ,cmax tmax and all that parameters.

21 September 2013 7,317 8 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I improve the quality of lamb milk replacer for machine feeding?

Hello everyone, I am researching ways to enhance the quality of lamb milk replacer for machine feeding. I would appreciate any insights or recommendations on the following: Manual vs. Machine...

06 August 2024 6,227 2 View

I found the zeta potential of exosomes in DLS and it was -8mv. So is it correct or not ?

What should be the zeta potential of bovine milk exosomes

25 July 2024 7,376 5 View

How does the human microbiome influence health and disease, including implications for personalized medicine and microbiome-targeted therapies?

Probiotics related.

22 July 2024 1,707 2 View

How can microbial engineering techniques be used to enhance the functionality and efficacy of probiotics ?

through genetic modification or metabolic engineering? Is it helpful, good for health ?

22 July 2024 7,811 4 View

What role does the gut microbiome play in immune system modulation and disease susceptibility?

Only Probiotics Related

22 July 2024 6,454 0 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

How to ask to correct the Incorrect Claim regarding the writer of Article that I was involved in?

Dear sir/Madam, I am Parinaz Parsa the first writer of article "Optimisation of probiotic yoghurt production enriched with phytosterols" and understand that one person is mentioned as writer which...

14 July 2024 9,579 2 View

How to make chocolate milk ?

we want to produce chocolate milk industrally .Dose anyone have a specific stabilizer formula for this pourpose?

10 July 2024 3,791 1 View

Many scientists have proven that the use of antibiotics against pathogens in dairy cows has a low success rate. What is the solution ?

I think that in such situations, hygiene measures are very important and the right milking machine is required. Which is the right one to obtain clean and safe milk with a high quality standard?

10 July 2024 4,362 0 View

Mohanad Fadhl Hussain

i believe this may help you

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6138816/

Jasim Al-Saadi

Article Probiotic Potential of 3 Lactobacilli Strains Isolated From ...

Article Lactobacillus salivarius CECT 5713, a potential probiotic st...

http://www.docsdrive.com/pdfs/academicjournals/ijds/2008/194-199.pdf

Alaa Kareem Niamah

Hi,

Milk and fecal (1to3 months) samples is good source of probiotic bacteria. you can isolate from this samples by selective media such as NNLP-MRS for Bifidobacter or MRS-sulicin for Lb. acidophilus. The optimum condition of probiotic is 37C for 72hours under anaerobic growth.