Detergent contains sodium laurel sulfate, which cleans dishes by removing fats and proteins. It acts the same way in the DNA extraction protocol, pulling apart the lipids and proteins that make up the membranes surrounding the cell and nucleus. Once these membranes are broken apart, the DNA is released from the cell
The isolation of DNA usually begins with lysis, or breakdown, of tissue or cells. This process is essential for the destruction of protein structures and allows for release of nucleic acids from the nucleus. Lysis is carried out in a salt solution, containing detergents to denature proteins or proteases (enzymes digesting proteins), such as Proteinase K, or in some cases both. It results in the breakdown of cells and dissolving of membranes.
Detergent contains sodium laurel sulfate, which cleans dishes by removing fats and proteins. It acts the same way in the DNA extraction protocol, pulling apart the lipids and proteins that make up the membranes surrounding the cell and nucleus. Once these membranes are broken apart, the DNA is released from the cell
Dear Dr.Alla
Detergent contains sodium laurel sulfate, which cleans dishes by removing fats and proteins. It acts the same way in the DNA extraction protocol, pulling apart the lipids and proteins that make up the membranes surrounding the cell and nucleus. Once these membranes are broken apart, the DNA is released from the cell.
The isolation of DNA usually begins with lysis, or breakdown, of tissue or cells. This process is essential for the destruction of protein structures and allows for release of nucleic acids from the nucleus
What is the function of detergent in DNA extraction?. Available from: https://www.researchgate.net/post/What_is_the_function_of_detergent_in_DNA_extraction [accessed May 8, 2017].
hello dear doctor Alla ...
isolation of DNA usually begins with lysis, or breakdown, of tissue or cells. This process is essential for the destruction of protein structures and allows for release of nucleic acids from the nucleus. Lysis is carried out in a salt solution, containing detergents to denature proteins or proteases (enzymes digesting proteins), such as Proteinase K, or in some cases both. It results in the breakdown of cells and dissolving of membranes.
What is the function of detergent in DNA extraction?. Available from: https://www.researchgate.net/post/What_is_the_function_of_detergent_in_DNA_extraction [accessed May 11, 2017].
https://www.researchgate.net/deref/https%3A%2F%2Fwww.labome.com%2Fmethod%2FDNA-Extraction-and-Purification.html
https://www.researchgate.net/deref/https%3A%2F%2Fwww.labome.com%2Fmethod%2FDNA-Extraction-and-Purification.html
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