Without the primer sequence it is not possible to determine the size of the fragments.

The primer sequences for rs2293152, is 5′-TAGAGGCTTCCTTTTGTTCCG-3′ (forward) and 5′-CCAGTTGTCTTTCATCCC-3′ (reverse). The primer sequences for rs744166 is 5′-GAGTACAAACCCTGAACC-3′ (forward) and 5′-GACTTGGTGACTGACTGAA-3′ (reverse) .

It is quite difficult to determine your fragment sizes correctly (for your specific study) with this limited information.

Still I am giving you some possible fragment sizes-

rs2293152 RE- AluI

PCR product - 356

Fragments- 195, 161

rs744166 RE- AciI

PCR product- 301

Fragments- 204, 97

rs744166 RE- AluI

PCR product- 301

Fragments- 98, 203

**The fragment sizes might be different if you consider the polymorphic allele.

Thank you.

Thankyou sir, one of the research article has given me 223 and 123 for 356 product size of rs2293152 , while for the other they have given 202 and 99

Sir you have mentioned the same enzyme Alul for the rs 2293152 as used for rs 744166? Is it possible?

You can give me the link of that article. So That I can check.

Rs 744166 of stat3 gene is associated with risk of gastric cancer in hans chinese population. N i have done little mistake in writing the fragment sizes for rs2293152 it is 223 and 133 while for rs744166 it is 202 and 99.

They hav not mentioned the fragment sizes in the article, i asked the corresponding authour and they have emailed me.

I just consider your primers and restriction enzymes for determining fragment sizes. That's why I have included all the fragments produced by these REs.

But obviously for specific rs you have to use specific RE.

Thankyou sir