I need some data about water containing bacteria at natural conditions. Which fast-testing method is most suitable find out if sample of drinking water is good or bad?
Dear Hazim,
You can isolate bacteria from water sample by different methods like direct streaking on selective and differential media, pour plate technique, and by filtration system.
https://www.researchgate.net/publication/273449505_Antimicrobial_Resistance_Profiles_of_Enterobacteriaceae_Isolated_from_Rosetta_Branch_of_River_Nile_Egypt
Article Antimicrobial Resistance Profiles of Enterobacteriaceae Isol...
To determine if water is safe to drink from a microbiological just make sure it is not contaminated by pathogenic microorganisms. But the latter belong to many different genera and species that their research is long and laborious; So you prefer to search for the so-called "indicators of faecal contamination" that some species of bacteria (coliform and fecal streptococci) present in large numbers compared to pathogens in human feces and animals. The presence of these bacteria "indicators" in water indicates fecal contamination and the suspected presence of pathogens, therefore, is to consider not drinking.
Essentially be excluded the presence of:
- Helminths (worms) such as Schistosoma (larva) or other helminths as Fasciola hepatica (larva), Taenia solium (eggs), Echinococcus (eggs)
- Protozoa such Entameba histolitica and Giardia intestinalis and Cryptosporidium parvum
- Bacteria: Salmonella typhi and paratyphi A and B, other Salmonella (various species), Shigella (various species), Yersinia enterocolitica,
Escherichia coli (enteropathogenic), Campylobacter jejuni,
Vibrio cholerae
- Virus: Adenoirus, Echovirus. Norwalk virus, Hepatitis A, Hepatitis E
This set is almost complete.
FGood for water a quick indicator, you may filter trhough 0,2 micrometers membrane. Cut it in two and seed half in nutritive agar and half in Levine agar Not good for heave contaminated waters.
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