What is the extinction coefficient of NADH and NAD+ at 280 nm?

Atilio Reyes Romero @Atilio_Reyes_Romero

01 January 2017 0 10K Report

I have an enzyme that has NADH. I don't know which form (protonated or not) is bound to the protein or if the co-factor is inside the enzyme.

How is possible to determine these forms? Any hits to determine at 280 nm in the isolated protein?

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