I have an enzyme that has NADH. I don't know which form (protonated or not) is bound to the protein or if the co-factor is inside the enzyme.
How is possible to determine these forms? Any hits to determine at 280 nm in the isolated protein?
03 April 2017 8,722 4 View
I am pretty new in the field of protein purification. Normally I use AHT (anhydrotetracycline) to induce E coli BLR strain and p-ask-IBA3 as vector. Is there any difference between using BLR and...
11 December 2016 7,496 4 View
I would like to get the binding energy of water molecules. Does anybody know a free software or a way to do it (such as is pymol)?
09 October 2016 3,318 0 View
08 September 2016 9,112 4 View
What I normally do is get the protein primary sequence from the PDB and I order the right sequence of the DNA from the company. Any suggestion/tutorial?
08 September 2016 7,635 4 View
24 February 2021 4,833 3 View
Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...
22 February 2021 7,801 3 View
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
21 February 2021 5,379 2 View
Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...
14 February 2021 2,610 1 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
I need to determine the open formula of our complex, presumably consisting of sodium tungstate Na2WO4 and aluminum sulfate Al2 (SO4) 3. Our problem is that with an increase in the concentration...
11 February 2021 5,821 1 View
I'm looking for advice regarding what effect size to use for meta-analysis. Our question is whether the number of individuals in a group affects the per-capita productivity of that group. Does...
10 February 2021 5,510 8 View
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
08 February 2021 7,285 1 View
07 February 2021 2,006 1 View
Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...
06 February 2021 2,847 2 View