Does a macrocycles database exist for fishing some knowledge from nature and from the drugs already in use in the market?

03 April 2017 8,722 4 View

What is the difference between BLR and BLR (DE3)?

I am pretty new in the field of protein purification. Normally I use AHT (anhydrotetracycline) to induce E coli BLR strain and p-ask-IBA3 as vector. Is there any difference between using BLR and...

11 December 2016 7,496 4 View

Which computational software is suitable to calculate the binding energy of water molecules?

I would like to get the binding energy of water molecules. Does anybody know a free software or a way to do it (such as is pymol)?

09 October 2016 3,318 0 View

What is the most efficient method to make a complex of two molecules?

08 September 2016 9,112 4 View

How to design a DNA sequence from an already known protein one?

What I normally do is get the protein primary sequence from the PDB and I order the right sequence of the DNA from the company. Any suggestion/tutorial?

08 September 2016 7,635 4 View

Is there any teachers based in UK who would like to take part in my study?

24 February 2021 4,833 3 View

Interpretation of Goodman - Kruskal Gamma squared?

Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...

22 February 2021 7,801 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

How to determinate of molecular structure of salt into the liquid?

I need to determine the open formula of our complex, presumably consisting of sodium tungstate Na2WO4 and aluminum sulfate Al2 (SO4) 3. Our problem is that with an increase in the concentration...

11 February 2021 5,821 1 View

What effect size should i use for a meta-analysis when study data violate normality assumptions?

I'm looking for advice regarding what effect size to use for meta-analysis. Our question is whether the number of individuals in a group affects the per-capita productivity of that group. Does...

10 February 2021 5,510 8 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Electron density difference maps(Fo-Fc): what is the meaning of negative densities (red blobs)?

Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...

06 February 2021 2,847 2 View