What is the experimental setup to determine Ammonia in Gas by Rose Bengal?

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How to calculate the neutron flux using Geant4?

Is there any free software for caring out these calculations.

21 October 2023 4,961 0 View

I want to do cellulose,hemi cellulose ,pectine and lignin extraction from pineapple fruit flesh. Which methodology will be the best?

this will be done to knoe fibre composition

19 September 2023 8,199 2 View

I need a protocol to reduce any oxidized (Fe3+) hemoglobin in my sample to (Fe2+)?

Hi everyone, I’m using hemoglobin in the lab and I need it to be in the reduced state (so it is able to bind with CO2). The hemoglobin I have is a lyophilized powder from Sigma Aldrich (H7379)....

13 September 2023 9,133 4 View

Does anyone know the equilibrium constant of guanidium chloride to urea and then of urea to isocyanic acid?

Hello, I need to use guanidium chloride to denature a protein. However, I’m reluctant because it is absolutely crucial for my experiment that no isocyanic acid is formed. I need to know the...

13 September 2023 4,587 1 View

Hı!, I'm estimating a Panel VAR using the stat pvar package which estimates homogenous pvar. How can I get irf for individual country as well thanks?

Please suggest any other method for estimating Heterogenous Panel Vector AutoRegressive thus allowing individual irf to be computed.

12 June 2023 9,061 3 View

Can i use probenecid (water insoluble) in measuring intracellular calcium concentration from HIT_T15 cells?

Dear Researcher, I am working on HIT_T 15 cells and I want to measure the intracellular calcium concentration using Fura_2am, I found many protocols using probenecid , My question: is it ok if i...

17 April 2023 6,450 0 View

Can I transfare the cells from well to another well?

I made a mistake, I seeded my cells in 6 well plates instead of 96 well plates. my question is can I transfer my cells to 96 well by using trypsin is that possible?

12 February 2023 7,826 0 View

How do I prepared 0.25% (w/v) Trypsin 0.53 mM EDTA solution?

The molecular weight of EDTA is 292.24g/mol Thanks in advance!

01 February 2023 5,421 3 View

What is the proper protocol for [Ca2+]! concentration measurement ?

I'm working on peptides from frog skin secretions, and I need to measure the concentration of [Ca2+]! release from HIT-T15 cells. I'm confused about the proper protocol for [Ca2+]! assay by using...

12 May 2022 786 1 View

Where can i find mathematical derivation of Superconducting cavity detuning calculation using forward and cavity voltage signals??

Usually cavity detuning is found by cavity pick up signal and feedforward signal from waveguide directional coupler. Cavity RLC mathematical model leads to 2nd order differential equations and...

11 May 2022 2,000 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

What is the main used curing agent for epoxy based coating?

I am trying to coating metal oxide into steel substrate, for better sticking planning to utilse epoxy based, Could you please suggest suitable curing agent for the coating purpose

25 July 2024 438 3 View

Can we isolate microplastic from sludge sample without using vacuum filter..?

isolation of microplastic from sludge sample using centrifugation ..

23 July 2024 6,418 0 View

How do you get pure primary macrophage cell culture?

I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...

23 July 2024 4,074 2 View

Optimal condition for depositing FTO target 95:5% using sputter technique?

Hello I want to know about the sputtering condition of depositing FTO from target 95:5%. I tried with RF sputter in pressure 2.5Pa and 100 sccm Ar at room temperature but it showing no...

21 July 2024 1,680 2 View

How is surfactant addition calculated?

I want to make 100 ml of MWCNT/distilled water mixture at a volume fraction of % 0.1. MWCNT density is 2100 kg/m3. I will add gum arabic as surfactant. If I add surfactant at a 1:1 ratio, will the...

19 July 2024 8,494 0 View