Why my pcr product was not appearing after gel purification?

I am unable to get purified plasmid after the gel purification of my PCR amplified product(2kb size). I tried both ways like kit method and manual like I prepared my own plasmid binding buffer,...

24 September 2020 7,867 6 View

Can we fabricate strips of silicon nano wires, so that they can be used for capacitive pressure sensing applications at low pressures?

use of silicon nano wires in pressure sensing applications

06 February 2020 7,972 3 View

I am in search of vendors for SOI wafer with device layer thickness of 150nm, can anybody suggest me some vendors who can supplies these SOI wafers

I am in search of vendors for SOI wafer with device layer thickness of 150nm, can anybody suggest me some vendors who can supplies these SOI wafers

29 May 2017 6,957 1 View

How can I calculate absolute electronegativity of CuAl2O4?

09 April 2017 211 31 View

No title

CuAl2O4 and ZnAl2O4 nanoparticles have been studied for photocatalytic activity. While performed with methylene blue dye using visible light I observed degradation (color difference) but I could...

11 August 2016 6,329 6 View

Can we perform CVD after TMAH? if not can anybody suggest me alternate way to get L layer deposited after TMAH. we don't have RIE/DRIE so.

01 January 1970 1,373 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Can anyone provide the Support vector regression code structure of LIBSVM MATLAB or python ?

Please, if Gaussian Parameter optimization is possible in this code structure

28 February 2021 278 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

VIF very high, but correlation is low and coefficient is significant. Which one whould I choose?

Hello. I have a question whether if my coefficients (statistically significant) would be valid when VIFs are really high. In my model, there are independent variables(IV), moderating variables...

26 February 2021 956 13 View