How to increase the brain tissue integrity during the harvesting?

Dear Researchers, I am working on an animal study and I want to produce high quality of tissue imaging. The probelm, is that I have low quality of cryosectioning which can be noticed after...

11 December 2018 3,264 3 View

Transfection Problem with a patient derived cell line?

Dear Researchers, I am working with a PDCL cancer cells and I want to transfect GFP in the cells. I am using lipofectamine 2000 and I incubated it with the cells for 6 hour and seems to be good...

08 September 2018 671 4 View

What is the difference between HOESCT 3342 and H33258?

Hey All, I am trying to use the dye for visualizing the BBB opening. Can anybody assist me with an information about both dyes in regard of the practical issues and the reliabiltiy of the two...

07 August 2018 3,468 1 View

Could it be possible to have liposomal form of certain protein to be delivered to the brain?

Dear Researchers, From Pharmaceutical point of view: Could it be possible to have liposomal form of certain protein to be delivered to the brain or not? What is the practical issues related to...

10 November 2016 2,226 4 View

When would be necessary to test the cognitive improvement among both male and female mice?

Dear Researchers,I am working on a behavioral and molecular study in a transgenic mice model. I want to evaluate the improvement in cognitive ability among mice after using certain agents. I want...

10 November 2016 7,404 8 View

Is it important to give mice a break between different behavioral tests? How long should it be?

09 October 2016 7,624 4 View

How we usually determine the neurotoxicity of certain Antibody?

05 June 2015 3,352 4 View

What is the best way for assessment of protein levels of TGF beta1 in splenocytes after challenging with test compound and con A?

I am working on splenocytes derived from healthy murine to investigate the effect of some compounds on interleukins and cytokines. I am using Con A as the mitogen to stimulate the lymphocytes...

03 April 2015 2,241 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View