How to increase the brain tissue integrity during the harvesting?

Dear Researchers, I am working on an animal study and I want to produce high quality of tissue imaging. The probelm, is that I have low quality of cryosectioning which can be noticed after...

11 December 2018 3,264 3 View

Transfection Problem with a patient derived cell line?

Dear Researchers, I am working with a PDCL cancer cells and I want to transfect GFP in the cells. I am using lipofectamine 2000 and I incubated it with the cells for 6 hour and seems to be good...

08 September 2018 671 4 View

Could it be possible to have liposomal form of certain protein to be delivered to the brain?

Dear Researchers, From Pharmaceutical point of view: Could it be possible to have liposomal form of certain protein to be delivered to the brain or not? What is the practical issues related to...

10 November 2016 2,226 4 View

What is the exact dimensions of 6 months mouse's Hippo-campus ?

10 November 2016 7,703 1 View

When would be necessary to test the cognitive improvement among both male and female mice?

Dear Researchers,I am working on a behavioral and molecular study in a transgenic mice model. I want to evaluate the improvement in cognitive ability among mice after using certain agents. I want...

10 November 2016 7,404 8 View

Is it important to give mice a break between different behavioral tests? How long should it be?

09 October 2016 7,624 4 View

How we usually determine the neurotoxicity of certain Antibody?

05 June 2015 3,352 4 View

What is the best way for assessment of protein levels of TGF beta1 in splenocytes after challenging with test compound and con A?

I am working on splenocytes derived from healthy murine to investigate the effect of some compounds on interleukins and cytokines. I am using Con A as the mitogen to stimulate the lymphocytes...

03 April 2015 2,241 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View