How to increase the brain tissue integrity during the harvesting?

Dear Researchers, I am working on an animal study and I want to produce high quality of tissue imaging. The probelm, is that I have low quality of cryosectioning which can be noticed after...

11 December 2018 3,264 3 View

Transfection Problem with a patient derived cell line?

Dear Researchers, I am working with a PDCL cancer cells and I want to transfect GFP in the cells. I am using lipofectamine 2000 and I incubated it with the cells for 6 hour and seems to be good...

08 September 2018 671 4 View

What is the difference between HOESCT 3342 and H33258?

Hey All, I am trying to use the dye for visualizing the BBB opening. Can anybody assist me with an information about both dyes in regard of the practical issues and the reliabiltiy of the two...

07 August 2018 3,468 1 View

What is the exact dimensions of 6 months mouse's Hippo-campus ?

10 November 2016 7,703 1 View

When would be necessary to test the cognitive improvement among both male and female mice?

Dear Researchers,I am working on a behavioral and molecular study in a transgenic mice model. I want to evaluate the improvement in cognitive ability among mice after using certain agents. I want...

10 November 2016 7,404 8 View

Is it important to give mice a break between different behavioral tests? How long should it be?

09 October 2016 7,624 4 View

How we usually determine the neurotoxicity of certain Antibody?

05 June 2015 3,352 4 View

What is the best way for assessment of protein levels of TGF beta1 in splenocytes after challenging with test compound and con A?

I am working on splenocytes derived from healthy murine to investigate the effect of some compounds on interleukins and cytokines. I am using Con A as the mitogen to stimulate the lymphocytes...

03 April 2015 2,241 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View