I want know How can convert IC50 which performed by MTT technique in vitro study to optimal dose in experimental tumor model fpr example EAC bearing mice.
I don't think such an equation exists. For in vivo experiments, you need to understand the pharmacokinetics (PK) of the compound (i.e. how the concentration at the tumor site varies with time after dosing) and the pharmacodynamics (PD) (i.e. how the tumor cells respond to their time- and concentration-dependent exposure to the drug). These can only be determined by experiment.
For this purpose, it may be possible to replace the animal model with a hollow fiber "glass mouse" system if such a system is available at your institute. This can provide cleaner PK/PD data and reduce the use of animals.
As Adam said, such an equation does not exist.
You cannot convert an in vitro IC50 concentration into an in vivo dose.
You must determine the maximal tolerated dose (five doses: 5 - 10 - 20 - 40 - 80) in groups of 3 healthy mice each and carefully observe the behavior, the weight (to be measured 2x/week (Monday - Thursday) and the survival of each mouse during 28 days.
This can be done as an i.p. acute (single) administration.
Then, you have to perform a determination of the maximal tolerated dose under chronic acdministrations (5x/w (Monday-Friday) during 2 weeks = 5ix2w for rapidly growing cancers or 3x/3w (Monday - Wednesday - Friday) = 3ix3w for "less rapidly" growing cancers.
I can further help you if you provide me with mode details.
Best regards
Robert
You need to decide the route of injection because therapeutic doses change with changing the route. After that you must determine the LD50 value of your compound. Then you use 1/10 or 1/20 of the LD50 as a therapeutic dose
Yes , I also calculate the therapeuic dose from acute toxicity study and then take 1/10 and 1/20th of the dose
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