What is the maximum number of amino acid residues that can be missing from the N and C ends of mNeonGreen and moxNeonGreen fluorescent proteins without affecting fluorescence?
For avGFP and its derivatives, using deletion analysis, we have defined the minimal domain in GFP required for fluorescence to amino acids 7–229.(Deletions of the Aequorea victoria Green Fluorescent Protein Define the Minimal Domain Required for Fluorescence, 1997)