First try just tap water treatment, particularly imortant is the time period. A great part of washing products do not work.

If I understood your question correctly, you want to get rid of all the impurities, debris or rest of the culture medium and to keep the vitality of Candida at the same time

so I advise you to use Phosphate buffer saline (PBS, pH 7).

Hello Sahar

Some useful references 1

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=0ahUKEwjA8qiukdnYAhXKK8AKHbtsA14QFggqMAA&url=https%3A%2F%2Fcandidahub.com%2FHome-Remedy%2FWashing-with-Soap-and-Water-for-a-Yeast-Infection&usg=AOvVaw3D4p048yZfoLXvA5_-FG2O

2

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=2&cad=rja&uact=8&ved=0ahUKEwjA8qiukdnYAhXKK8AKHbtsA14QFggwMAE&url=https%3A%2F%2Fmsu.edu%2F~eisthen%2Fyeast%2Fcauses.html&usg=AOvVaw2efMl0iadjv4Dpx8v7aCO0

3

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=3&cad=rja&uact=8&ved=0ahUKEwjA8qiukdnYAhXKK8AKHbtsA14QFgg1MAI&url=http%3A%2F%2Fbeatcandida.com%2Fwhats-the-best-intimate-wash-for-a-yeast-infection%2F&usg=AOvVaw2n4d93iSpDBZ3-a5mhOJxa

4

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=4&cad=rja&uact=8&ved=0ahUKEwjA8qiukdnYAhXKK8AKHbtsA14QFgg9MAM&url=https%3A%2F%2Fwww.self.com%2Fstory%2Fyeast-infection-causes&usg=AOvVaw1gk2Cp3iy_qqVWvQM6QgRb

5

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=5&cad=rja&uact=8&ved=0ahUKEwjA8qiukdnYAhXKK8AKHbtsA14QFghDMAQ&url=http%3A%2F%2Fwww.medic8.com%2Fhealthguide%2Farticles%2Fvaginalthrushproducts.html&usg=AOvVaw0rm25WypAmWRcGGb4Q89xg

6

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=6&cad=rja&uact=8&ved=0ahUKEwjA8qiukdnYAhXKK8AKHbtsA14QFghJMAU&url=https%3A%2F%2Fwww.thecandidadiet.com%2Fginger-antifungal%2F&usg=AOvVaw3Gm3LhAEJHbrcN_bCgMNnu

7

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=7&cad=rja&uact=8&ved=0ahUKEwjA8qiukdnYAhXKK8AKHbtsA14QFghPMAY&url=https%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles%2FPMC4881145%2F&usg=AOvVaw2Aat4kB-lxJOypbun76TfY

8

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=8&cad=rja&uact=8&ved=0ahUKEwjA8qiukdnYAhXKK8AKHbtsA14QFghYMAc&url=http%3A%2F%2Fwww.dailymail.co.uk%2Fhealth%2Farticle-2050239%2FHow-washing-machines-familys-health-risk-Low-temperatures-mixed-loads-spreading-dangerous-bugs.html&usg=AOvVaw0j2P-MZ5jQlAU2lOibSGP8

9

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=9&cad=rja&uact=8&ved=0ahUKEwjA8qiukdnYAhXKK8AKHbtsA14QFgheMAg&url=https%3A%2F%2Fpatient.info%2Fhealth%2Fvaginal-thrush-yeast-infection&usg=AOvVaw0t1FCvKYKDNVRyA215Z5PZ

10

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=10&cad=rja&uact=8&ved=0ahUKEwjA8qiukdnYAhXKK8AKHbtsA14QFghlMAk&url=https%3A%2F%2Fwww.healthdirect.gov.au%2Fvaginal-irritation-and-infection&usg=AOvVaw1kzlZ9pk5fV2PbObL-z_Oi

11

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=12&cad=rja&uact=8&ved=0ahUKEwjhksDektnYAhUDJpoKHat3A0c4ChAWCCwwAQ&url=https%3A%2F%2Fwww.sciencedirect.com%2Fscience%2Farticle%2Fpii%2FS1991790211000808&usg=AOvVaw0xljnX_bCNKyxmdfhV7B1E

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=13&cad=rja&uact=8&ved=0ahUKEwjhksDektnYAhUDJpoKHat3A0c4ChAWCDEwAg&url=https%3A%2F%2Fwww.yeastinfection.org%2Fis-listerine-good-for-yeast-infection%2F&usg=AOvVaw23_L9QZKhxvbRoAvP7mCQC21

13

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=14&cad=rja&uact=8&ved=0ahUKEwjhksDektnYAhUDJpoKHat3A0c4ChAWCDgwAw&url=https%3A%2F%2Fwww.myvmc.com%2Fdiseases%2Fvaginal-thrush-yeast-infection-vulvovaginal-candidiasis-vvt-vvc%2F&usg=AOvVaw2kjTM0rGIN5PeGOCUVvrUl

14

https://books.google.iq/books?id=QLin14nRVBsC&pg=PA139&lpg=PA139&dq=effect+of+washing+product+against+candida&source=bl&ots=CxlQqfA7x6&sig=iTgLnqQPDNyycdaEkU-CNX74Fyc&hl=en&sa=X&ved=0ahUKEwjhksDektnYAhUDJpoKHat3A0c4ChDoAQhEMAU

15

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16

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=18&cad=rja&uact=8&ved=0ahUKEwjhksDektnYAhUDJpoKHat3A0c4ChAWCE4wBw&url=https%3A%2F%2Fwww.healthline.com%2Fhealth%2Fskin%2Fcutaneous-candidiasis&usg=AOvVaw24BDbpvM1O6Sd_q0MC_18y

17

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=19&cad=rja&uact=8&ved=0ahUKEwjhksDektnYAhUDJpoKHat3A0c4ChAWCFQwCA&url=https%3A%2F%2Fwww.hse.ie%2Feng%2Fhealth%2Faz%2FC%2FCandidiasis%2C-women%2FTreating-vaginal-thrush.html&usg=AOvVaw1o38szejj9SS7QCnsjegrQ

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=20&cad=rja&uact=8&ved=0ahUKEwjhksDektnYAhUDJpoKHat3A0c4ChAWCFkwCQ&url=https%3A%2F%2Fwww.medicalnewstoday.com%2Farticles%2F246615.php&usg=AOvVaw1QLZesdBn5eALQMRNoJSCk

19

https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=24&cad=rja&uact=8&ved=0ahUKEwjI44jDlNnYAhVEKcAKHd11B-k4FBAWCDYwAw&url=http%3A%2F%2Fhealthywa.wa.gov.au%2FArticles%2FS_T%2FThrush-genital&usg=AOvVaw0t5Sen4NbRGvMWylspkf2q

20

https://books.google.iq/books?id=gQcAAAAAMBAJ&pg=PA46&lpg=PA46&dq=effect+of+washing+product+against+candida&source=bl&ots=NTzRwR_uhO&sig=KZEzRsarFg42tYTeBt6QtBsDfas&hl=en&sa=X&ved=0ahUKEwjI44jDlNnYAhVEKcAKHd11B-k4FBDoAQg8MAQ