I am doing an ouchterlony double diffusion test (ODD test) using a kit. It suggests 2 hours of incubation, or at room temperature overnight. Isn't diffusion faster at room temperature? I kept the plates in petriplate with wet cotton to maintain the moisture condition.
As per the collision theory, faster diffusion will take place if the surroundings are warmer. The speed of a molecule is proportional to the square root of the absolute temperature and temperature is the measure of the average kinetic energy of molecules in an object, therefore when the temperature increases the molecules will have more kinetic energy, so they move faster and due to more spontaneous spreading of the material diffusion occurs quicker. When this happens the rate of diffusion also increases, the opposite will happen if the temperature decreases. The Brownian motion of the molecules increases with temperature, and therefore the diffusion.
Thank u dear sir ......Yogesh Tripathi.
m expecting diffussion faster at room temperature compared to at 4 degree,but in that kit it was given that diffussion is faster at 4degree so i got confused i thought there may be other reason behind it.
I observed my ODD slides......I got my results ,as m expecting diffusion faster at room temperature than at 4 degree.i got good result in the slides which i kept at room temperature and i dint get any result in the slides which i kept at 4 degree.
- Badges
- Science topic
- Similar topics
- Linguistics
- Composition Studies
- Publication