In what temperature we should store a lyophilized serum sample for long term greater than one year? Is there any effect from keeping it in -20 C?
Dear Bhagya,
In my opinion, water is the main reason of destroying biological body, biological organ, biological tissue and biological molecules destroying and instability.
Omitting of water or making it inaccessible for the biological tissue, ...and biological molecule, is a reasonable method for long term storage of samples.
With lyophilizing we extract the water of sample as much as possible.
For next step, inaccessibility of the remain small amount of water is good strategy.
Freezing, with fixing the hydrogen bond among water molecules, make it inaccessible for any undesirable biological, physical or chemical reactions.
So, it means, storage of lyophilized sample in deep freeze such as -80 C is a good suggestion.
Of course lyophilized samples can keep at room temperature, at 2-8 C, at -20 C and -80 C. Walking from RT to -80 C for lyophilized sample, increase the sample stability.
best
Mehdi
We keep our samples at -85 C and we didn't see any effect on our samples.
Murat,
Yes as I mentioned -80 C or lower is suitable.
It can be stable in higher temperature such as -20 C. but in shorter time.
Murat,
it is important to take into account that, before to decide for the best storage condition, a good lyophilization process implies to remove water from biological samples in amounts higher than 96%.
Before freezing add poly ols (up to 3%) to the serum then freeze it and lyophilize it. The lyophilized sera can be kept at -20 degree centigrade for years together.
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