Bhagya C. Thimmappa

12 Questions 27 Answers 0 Followers

Questions related from Bhagya C. Thimmappa

Does anyone know the composition of Gelzan™ CM Gelrite® from Sigma-Aldrich?

Hello everyone, I am working with cranberry plants, and I grow them with plant growth media containing Gelzan. Growth media that I am using for my experiments contain different percentages of N,...

04 June 2021 3,009 3 View

Why I am getting same ct values for three different dilutions?

I am setting up qPCR with human DNA and actin primers, it is working with 10 and 1ng dilution but when I am considering 0.1, 0.01 and 0.001 I a getting same ct values for these 3 dilutions, I am...

07 September 2015 6,849 5 View

Hi, how to subculture transconjugants with out affecting the stability of the transconjugants?

After getting transconjugants, I am trying to subculture but there is no growth. Out of 5 different transconjugants only 3 are growing. Is it like transconjugants loosing the stability so they are...

12 August 2015 6,591 4 View

How do we account for overlapping genes and gene within gene? What technology is available to predict these kind of genes?

How do we account for overlapping genes and gene within gene? how r we going to predict those overlapping and gene within gene?Can we use any special gene prediction algorithms ? I am new to this...

02 November 2014 7,094 3 View

In gene prediction algorithms are we taking count of enhancers?

I feel the information about enhancers helps us to predict the gene properly, if a gene function is influenced by enhancers then why we r not counting enhancers while predicting gene? some one...

01 October 2014 694 5 View

Is there are any other modified bases in case of T-even Bacteriophages other than Methylation and glucosylation?

Biological DNA modifications have been studied for many years, and much is known about their distribution and the enzymes involved. Well-known base modifications are C-5-methylcytosine (m5C),...

17 July 2014 9,083 1 View

Hi everyone does anyone have protocols for the following tests?

Acid produced from: Glucose,D-Mannitol,Salicin,Inositol,Sorbitol,Rhamnose,Maltose,D-XYIOX,Trehalose,Cellobiose,Methyl D-glucoside,Erythritol,Melibiose,D-Mannose. Hydrolysis of: Aesculin,...

07 July 2014 5,144 9 View

Can anyone tell me about immunity and normal microflora?

Why our body fails to identify normal microflora as antigens?

16 February 2014 1,047 7 View

Can anyone tell me about reverse gyrase and hyperthermophiles?

I wanted to know about the lab or institutes where work on above topic is going on and the status of the research.

11 February 2014 8,087 6 View

What is the effect of temperature on diffusion?

I am doing an ouchterlony double diffusion test (ODD test) using a kit. It suggests 2 hours of incubation, or at room temperature overnight. Isn't diffusion faster at room temperature? I kept the...

04 February 2014 1,718 3 View

Can anyone tell me the mechanism of action of CTAB on the plant cell wall?

I mean how is it going to precipitate the polysaccharides? Does it precipitate +ve or -vely charged proteins? What about polysaccharides?

21 January 2014 3,725 9 View

What do methylated bases do in case of tRNA(phe)?

In the case of yeast tRNA (phenyl alanine) there is a G-U base pair (G4-U69), and many bases are methylatedlated like G26(2'methylated), C49(5'methylated), C40(5'methylated), and so on. If we look...

28 November 2013 2,649 2 View