How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Does the phylogenetic tree making tool IQTREE use evolutionary placement algorithm?

For my master thesis I am conducting a phylogenetic research and for this I'm using tools like MEGA, IQTREE, FigTree, etc to make a phylogenetic tree. I wanted to know if IQTREE integrates the...

14 February 2021 1,541 2 View

Is there an alternative to PGLS that considers phylogenetic signal when calculating the estimated value?

I have been working on a study trying to estimate body mass in mammals using a skeletal proxy in R. My dataset has high phylogenetic signal (Pagel's Lambda = 0.9) and I am interested in removing...

26 January 2021 6,997 3 View

When making a phylogenetic tree, do I need to concatenate the chromosome and the plasmids for my reference?

This is such a basic question but I come from a non-bioinfo group so I keep getting different answers. I was wondering if when making a phylogenetic tree whether you have to concatenate the...

14 January 2021 2,492 2 View

How can I build one phylogenetic tree for an arthropod community when I had known their scientific names?

I am working on the phylogenetic analysis for communities of plants and arthropods. A standardized protocol of building phylogenetic tree for plant species list that was widely used in studies....

29 December 2020 181 3 View

Are phylogenetic independent contrasts still used in biology?

I have a regression equation in which I am curious if the amount of scatter around the regression line is driven by phylogeny. I know there is a lot of phylogenetic signal because Pascal's lambda...

22 December 2020 937 1 View

Where Can I Get Hourly Wind Speed Data at 50 Meters Height?

Hi, I want to get three years of hourly wind speed data at 50 meters height for specific locations in Turkey. Unfortunately, I couldn't find any good websites for this. Do you know any online...

12 December 2020 323 10 View

Could anybody provide insights the best tools to create a custom database for taxonomy assignment of metagenomic data?

I am interested in the different tools that can be used to create custom databases for targeted sequencing and how to trim the databases based on the amplicon size? Also, should custom databases...

02 December 2020 3,105 3 View

How can I get Genbank accession code given the scientific names?

Hello! I now have a complete list of about 23k plant species that I want to check if Genbank has the sequencing data for a phylogenetic analysis later, and I am really new to the topic. Is...

02 November 2020 4,450 3 View

Can you recommend me tree databases?

Hi. I have slight experience in searching phylogenetic trees, normaly I use TreeBASE or Dryad but some papers that I found doesn't have the resulting trees in these databases or there are genetic...

26 October 2020 582 3 View