Would anyone advice on a procedure to check the effect of organo phosphates on Cholinesterase, using mice? Basically trying to ascertain the clinical effect of organophosphate contaminated foodstuff.
Is anyone working on or has experience with determining CREB and/or pCREB using western blot? Any tips on how I can get better bands of pCREB? I have no problem with CREB, but I always have very...
08 September 2012 7,911 10 View
Does is it really matter if the ECL reagent 'in the dark bottle' is exposed to light prior to mixing with the other solution? Am trying to figure out why am not getting the bands, when previously...
08 September 2012 1,374 18 View
I want to measure serum Ghrelin levels in mice samples. I collected serum about 3 weeks ago, and froze it at -80 degree. I only received the ELISA kit 2 days ago. The kit says the sample should be...
05 June 2012 5,354 7 View
Does depression really induce c- fos production in experimental animals? Anyone knows a research proving this.
03 April 2012 2,490 2 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...
02 March 2021 6,865 1 View
In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...
25 February 2021 5,229 3 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.
22 February 2021 7,800 3 View
I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...
22 February 2021 4,374 3 View
I am looking for something similar than Ingenuity Pathway Analysis (IPA) in which I can enter the proteins differentially phosphorylated and I can visualise the pathways these proteins are...
21 February 2021 8,923 4 View
I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...
18 February 2021 6,896 3 View
Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....
18 February 2021 5,109 5 View
Hello everyone, hope you are well. I am planning to use a Generalized Linear Model in SPSS. I would like to add gender to the model as a covariate, however from what I have read, only continuous...
14 February 2021 8,937 4 View