Recently, I conducted an experiment on maize under control conditions in white sand with two treatment variables ( 1; irrigated with distilled water and 2; irrigated with 60mM NaCl solution) and 40 replications. After three weeks I collected the data of SPAD from the first large leaf of each replication. I observed that 60mM NaCl treatment significantly increases the SPAD value with respect to control (treated with distilled water). Is it normal?
I think it might be due to the salt tolerance of maize, may also be due varietal effect
The morpho-biochemical response varies with the type of genotype used. Also, check the threshold salinity level of the maize crop.
No its not normal. The maize cultivars that you are using are sensitive/tolerant to salinity stress.
Muhammad Shahid Iqbal Dear I used the local seeds of maize (Origin Country: Yemen).
You must repeat the experiment with some replications, try to select same type of leaf, preferably 3rd or 5th from upper side which is available same for all samples, and replications and get average, If the result is same as earlier be confident otherwise update
The sensitivity of maize to salinity is associated with higher accretion of Na+ in the leaves (Fortmeier and Schubert 1995). A saline level of more than 0.25 M NaCl damages maize plants and may stunt growth and cause severe wilting (Menezes-Benavente et al. 2004).
As it is stated in one of the comments that 0.25 M sodium stress ll affect the plant (Maize) growth and your treatment is much lower (0.06M). This means it shall not have much effect on the Maize if you want to have a much clear picture then should run the experiment with some much lower and higher level. This can give you the stress level of the local variety for future recommendations. Moreover, if the germination index and other parameters are normal then the plant is good in NaCl uptake and the tolerance level can only be checked if your variety has not good germination and other parameters (height, leaf, stem, and root length, etc). I think the variety you are using has very little tolerance to NaCl, Hope you get good results.
Dear apply different concentrations (0, 50, 100, 150 and 200 mmol) of NaCl. Also perform triplicate experiment and note important morpho-biochemical and molecular parameters at different plant growth stages.
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