Has anyone tried cross-linking cells with formaldehyde before co-immunoprecipitation?

Hi, I am currently trying to co-immunoprecipitate a protein but the interaction between that protein and its binding partner is not stable. Sometimes we able to detect its binding partner but...

29 November 2020 9,261 3 View

How to fix half the molecules in an ensemble while annealing?

I created a rectangular ensemble consist of crystalline erythritol molecules. I want to create a liquid-solid interface through annealing, making half of the ensemble amorphous and keep the other...

27 September 2020 2,632 1 View

GROMACS How do I apply position restraints on erythritol molecules?

I was trying to apply restraints on my molecules, however, they were still moving after I put 100000kJ/mol/nm^2 on them. I am sure my topology is correct, so I think maybe its the correlation of...

23 September 2020 3,958 3 View

TOP/FOP-FLASH assay in HEK293T cells?

Has anyone tried to study Wnt signaling in HEK293T cells? I have been trying to measure the Wnt activity using TOP/FOP-FLASH assay but the readings that I got are rather similar in both TOP and...

28 August 2020 6,052 4 View

A question about western blot?

I have run western blots for 25 years. Today I got this strange blot using Aviva OAPB01225 klotho antibody and 1% BSA in TBST to block for 1 hour. It doesn't matter whether I load 10 ug or 50 ug...

31 December 2019 9,605 3 View

Loading control for membranbe proteins?

I need to study cell membrane protein by western blots but encounter a problem with adequate loading control. Any suggestions?

07 August 2019 3,010 5 View

How mitochondria mediates fatty acid metabolism in cancer cells?

We just identified a mitochondrial protein that regulates fatty acid metabolism (FAM) in cancer cells. Does somebody have expertise in FAM? For example, which kit(s) or method(s) can be used to...

01 January 2019 4,187 3 View

Calculation of drugs concentration from IC50(in vitro) to CED(in vivo) injection?

Convection-enhanced delivery (CED) system is a powerful treatment for directly bypass BBB in brain tumor therapy. I try to set up the system and the in vivo animal model. In the begin, we use TMZ...

26 June 2018 7,719 3 View

Do we have unimolecular organic reaction during 350K for kinetic study?

30 January 2018 7,970 2 View

Any need to do unit root test for very short time series?

I read that the validity of unit root test becomes very small if the number of observations is small. In this case, it is very likely to have Type II error, say wrongly accepting the null...

08 January 2018 1,505 11 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View