What should be the number of cells per well required for MTT assay (cell growth inhibition assay)?

What should be the optimal number of cells per well in a 96 well plate to perform cell growth inhibition assay or MTT assay? The cell line in question is the A-431 cell line. The cell line has a...

31 October 2019 1,763 4 View

Quantification of Asparagine using HPLC ?

I am trying to quantify asparagine in my fermentation broth using HPLC, C18 column with mobile phase 0.1% tfa: Acetonitrile (95:5 ). I'm not getting a perfect peak with the standard. The...

07 January 2018 3,050 5 View

Increase in pH with aeration in cell culture bioreactor?

09 September 2016 9,260 3 View

How to control gas flow for culturing CHO cell in reactor?

we want to maintain 5% co2 inside the fermentor, for that what condition are needed. eg what flow rate of co2 or o2 or air to be supplied. or anyone here used fermentor for mammalian cell culture...

26 June 2016 6,454 1 View

How do I distinguish HE and EH type modes in exact analysis of multilayer circular waveguides?

When we study the exact mode analysis of circular waveguides (Ref. Light Transmission Optics by D. Marcuse and Fundamentals of optical waveguides by Okamoto), we get an eigen value equation. If we...

24 March 2015 8,072 3 View

How do I distinguish HE and EH type modes in exact analysis of multilayer circular waveguides?

When we study the exact mode analysis of circular waveguides (Ref. Light Transmission Optics by D. Marcuse and Fundamentals of optical waveguides by Okamoto), we get an eigen value equation. If we...

02 March 2015 1,694 4 View

Why did half of the capacitor charging energy go in parasitic resistance irrespective of resistance value ?

I know half of that energy is dissipated in heat in the resistance of the charging pathway, and only QVb/2 is finally stored on the capacitor at equilibrium I'm just going to lower the resistance...

14 February 2015 362 12 View

On what basis do we differentiate nanowires from nanorods?

Is it the aspect ratio (Diameter/Length), if yes then what is the range of value of Aspect ratio that defines nanowires and nanorods?

08 September 2014 8,609 15 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View