How to stain bacteria with propidium iodide to determine dead cells by flow cytomatory and microscopy?

I have to determine the dead cells in culture and I have propidium iodide in powder form and I have diluted 2mg with 2mL sterile filtered double Distilled water and to stain bacteria I use 10micro...

03 August 2020 8,934 4 View

NMR not matching

I have a compound. Its Mass spectra is matching with the structure.And in HPLC is also matching with the reported RT. But I m getting almost double the number of protons in aromatic region in NMR....

17 February 2016 1,981 3 View

How can I find base shear, overturning moment, story drift and lateral displacements in SAP2000 using response spectrum analysis?

08 November 2014 4,998 6 View

How can I perform segmentation in image processing?

I want to apply segmentation by first identifying one whole line from the image then identifying words and letters from it.

24 April 2014 2,055 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Do you kow of any database containing data on retention time, log P and polarizability for a large number of molecules?

Hi, I am a chemistry undergraduate doing my dissertation project on HPLC. In my project, I need to predict the HPLC retention time of 20 aromatic molecules, the calibration set. To do this I will...

24 February 2021 2,602 1 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

How to analyse monosaccharide compositions from soluble fiber?

I have tried to hydrolyze the sample using 2 M TFA at 100C for 24, 48 h and 120C for 2 h. From these conditions, the HPLC results showed that peaks were not separated into monosaccharide. I found...

17 February 2021 5,230 1 View

No title

Hello. I ask for help - I need to get lithium tetraborate (soluble salt) from lithium carbonate and boric acid. In literature, everything seems to be simple - mix, ignite and - it's done. But it...

16 February 2021 9,523 2 View

Is There are any idea to achieve H NMR signals for swollen compound ??

the samples are not completely soluble - just swelled the solvents that's mean the sample may be partially soluble any suggestion, please

16 February 2021 8,801 3 View

Which non-coordinating solvent dissolves anhydrous Iron(II)chloride?

Hallo, i am looking for a non coordinating solvent that dissolves anhydrous FeCl2 for a synthesis in which i can not use solvents like thf, pyridin etc.. I already tried benzene (no solubility),...

11 February 2021 6,105 1 View

DMSO concentration in cell culture? Precipitating while PBS is added?

I am testing a compound on RAW 264.7. the compound is soluble in DMSO. but to ensure that final concentration of dmso is not more than 0.1%, we are first dissolving it in dmso and then diluting it...

11 February 2021 1,136 3 View

Does Gaussian 09W software take too long to give a result if we have a large chemical structure ?

DFT - gaussviev - gaussian09W - HOMO - LUMO

08 February 2021 6,012 5 View