Hi Abdi Ali!
The CLED agar is a non-inhibitory growth medium, therefore any growth in the media will mean the urine is either positive, or the sample was contaminated. That’s why it’s recommended that you inoculate the medium immediately after the urine collection.
This media is mostly used for the isolation and differentiation of urinary organisms. Bromothymol blue, the indicator used in this agar, changes to yellow in case of acid production during fermentation of lactose, showing Lactose-positive bacteria as yellow colonies. Bacteria which decarboxylate L-Cystine cause an alkaline reaction and build deep blue colonies.
Its electrolyte deficiency prevents the swarming of Proteus species, also Shigella species may not grow on this media. When the sample is collected after an antibiotic therapy or the urine pH is low, it may suppress the growth and give a lower bacterial count.
I hope this answer will be helpful, and if you have any other related questions don’t hesitate to contact me.
Kind regards, Diego.
the Diego´s answer is correct, but you have to using the Kass´s Method to determine the degree of infection
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