Because keratin contains very sable secondary structures of amino acids..... I referred to this paper (Kakkar et al. SpringerPlus 2014, 3:596) and I think this would be really helpful.

The first step of extraction is defatting i.e. removal of fats

from the raw material. Soxhlet’s apparatus was used to

carry out defatting/delipidization of pulverized hoof sample

for about two days. Mixture of hexane and dichloromethane

(1:1, v/v) was used for refluxing. Ten gram of

defatted hoof sample was mixed with 7 M urea, 6 g SDS

and 15 ml of 2-mercaptoethanol in a 300 mL roundbottom

flask and kept in orbital shaker at 60°C for 12 h to

extract keratin at pH 7. The resulting solution was then

centrifuged for 15 mins at 6,000 rpm and the supernatant

was dialyzed against degassed water for 5–6 days. Some of

the extracted keratin was kept in a deep freezer at −80°C

for 5 h and lyophilized to make it into powder. Hereafter,

the dialyzed keratin i.e. prior to lyophilization will be mentioned

as liquid keratin and the powdered one will be

mentioned as lyophilized keratin.

I recommend the “Shindai method” to extract proteins from mammalian hair. This method delivers a high yield of solubilized proteins by using thiourea (2.6 M) and urea (5 M). The solubilized proteins consisted of keratin and KAPs.

Please find the papers for your reference

Best luck

https://www.researchgate.net/publication/317500618_Characterization_of_keratin_microparticles_from_feather_biomass_with_potent_antioxidant_and_anticancer_activities

https://www.researchgate.net/publication/317328078_Statistical_investigation_of_extraction_parameters_of_keratin_from_chicken_feather_using_Design-Expert

https://www.researchgate.net/publication/316467223_Dissolution_and_characterization_of_biofunctional_keratin_particles_extracted_from_chicken_feathers

https://www.researchgate.net/publication/301810903_Sustainable_Management_of_Keratin_Waste_Biomass_Applications_and_Future_Perspectives

Article Characterization of keratin microparticles from feather biom...

Article Statistical investigation of extraction parameters of kerati...

Article Dissolution and characterization of biofunctional keratin pa...

Article Sustainable Management of Keratin Waste Biomass: Application...

Thank you Ashok.... Everything I need I guess....very helpful.

Welcome

feel free to contact if any quarry

regards

Definitely I will. Wish u success in your pursuit of knowledge

https://www.researchgate.net/publication/329177982_Keratin_as_a_Protein_Biopolymer_Extraction_from_Waste_Biomass_and_Applications?_sg=vT3AETuRFa-XoZ08cNLnhpM4CfD_rgHVGQpiP7I_aTXQNRJK6aiS5lBuPx2UZblQ6_vF4LXTHwjej85bfcvNfkGk6LdZ9yx0rrEhJfoN.uDFGxmUJGl7DzXTT79Ta_FdcO2ZRd_ue03f5x4DoXO1P38Vyr3pO4YiKc3Yf7LD9yCQX0MMyFbiMJzlkRtTOqQ