On downloading protein sequence records from the NCBI, what is the difference of the GenPept format ant the GenPept (full)?. Is there any differences in which sequences are included, or is the difference only in the formatting?
Exism movements after gaining power within liberal democracies under majority rule and independent rule of law system become permanent dictatorship threats, but why this is the case is not clear...
04 August 2024 8,125 3 View
Since 2016 Brexit, the world needed to change the thinking behind traditional democracy as the democratic landscape changed, yet traditional democratic thinkers and actors have been acting as if...
28 July 2024 6,515 1 View
Perfect democracy thinking assumes no chaos so no need for independent rule of law system and liberal democracies assume the possibility of normal democratic chaos that can be sorted out by an...
28 July 2024 473 1 View
Exism movements since BREXIT 2016 have been described as driven by emotions leading to the idea of Emocracy/Emocracies, but as the social discontent that is usually displayed after exism movements...
16 July 2024 3,701 1 View
If you understand what is the necessary and sufficient condition for exism movements like Brexism, Trumpism, Brazilianism.... to persist at all cost in power once they have power are within a...
10 July 2024 8,048 1 View
You have seen the comings and goings now of Trumpism, Brazilianism, and Brexism, and the common theme is why they failed to persist in power ONCE THEY CAME TO POWER. If you look at the evolution...
06 July 2024 7,050 1 View
You have seen the comings and goings now of Trumpism, Brazilianism, and Brexism, 2016 to 2024 and the common theme is why they failed to persist in power ONCE THEY CAME TO POWER. If you look at...
06 July 2024 9,022 1 View
Are you concerned about the future of democracy, locally or globally? What do you think the fundamental lessons learned for democracy are since 2016 BREXIT? How can we come out with a...
06 July 2024 5,962 4 View
You have seen the comings and goings now of Trumpism, Brazilianism, and Brexism, 2016 to 2024 and the common theme is why they failed to persist in power ONCE THEY CAME TO POWER. You have seen...
06 July 2024 6,790 1 View
It seems that since 2012 Rio +20 Conference, the world has been moving away from science based perfect market thinking, a moved that apparently has been helped by the confusion created by the 2012...
02 July 2024 4,530 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Molecular docking software/ websites?
02 August 2024 8,704 7 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
30 July 2024 1,061 5 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View