How should the scholar working in the laboratory and in field based activities under a Project be handled by Principal Investigator?

All the externally aided projects which are funded have to go through rigorous checks and appraisals on a continued basis depending on the work plan. In order to meet the timeline the scholars...

31 May 2020 9,111 4 View

How can deforestation and anthropocene impact the biodiversity and lead to incidence of pest and diseases?

It has been alarming that human activities had increased many proportions and had led to many anthropogenic activities which had been directly and indirectly affecting the natural ecosystems....

29 April 2020 7,424 1 View

Why conventional breeding which has a much better role to play in genetic improvement of trees is not given much importance in research?

It is seen that conventional breeding is losing its charm in research as compared to modern biotechnological methods even though it is not preferred in terms of its field deployment. Moreover, if...

26 March 2020 3,225 20 View

IGD indicator, GD indicator python or julia (MOEA)?

Hello all, Does anybody know any framework/code for IGD indicator, GD indicator in python or julia programming?.Also Is it ok to use a hypervolume indicator when it is biased with respect to...

30 July 2019 2,087 3 View

What is flexion point of a polymer?

How the flexion point is related to the mechanical strength properties and degradation of a polymer?

27 February 2018 5,081 1 View

What types of cathode lamps has to be used in AAS ie. coded or noncoded lamps?

i has to purchase AAS lamps in that what should i will buy coded or noncoded cathode lamps. what is difference between these two.

30 November 2017 2,856 4 View

Can anyone one help on the issue regarding determination of peroxide value?

04 January 2016 9,921 2 View

Formula for calculating crystallite size and lattice strain using XRD data?

What is the standard procedure to calculate crystallite size and lattice strain using XRD data. How accuracy the Scherrer formula is. Can I use it to calculate crystallite size and also How about...

14 November 2014 7,864 10 View

How can one identify the topological switch point in proteins?

13 November 2014 9,357 1 View

Multiple ligand docking. Can anyone suggest software to perform multiple ligand docking studies?

Can anyone suggest software to perform multiple ligand docking studies?

06 October 2014 1,860 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View