What is the difference between reverse transfection “neofection” using siPORT™ NeoFX Transfection Agent and standard transfection? Can we use any of them for MicroRNA transfection in human cell line ?
Reverse transfection and standard transfection are two different methods used to introduce genetic material into cells, but there are differences between them.
In standard transfection, cells are first plated in a dish, and then a transfection agent and genetic material (such as plasmid DNA) are added to the cells. The cells are then incubated to allow the genetic material to enter the cells and express the desired protein or RNA.
In reverse transfection, the genetic material and transfection agent are mixed together and then added to the cells, which are then plated in the dish. This allows the cells to attach to the dish and start to grow before the genetic material is introduced.
Neofection, which uses the siPORT™ NeoFX Transfection Agent, is a type of reverse transfection that is optimized for RNAi experiments. The siPORT™ NeoFX Transfection Agent is designed to efficiently deliver small RNAs (such as siRNA or miRNA) into cells.
Both standard transfection and reverse transfection can be used for microRNA transfection in human cell lines. However, the choice of transfection method depends on the specific experimental conditions, such as the type of cells being used, the amount of genetic material needed, and the desired level and duration of gene expression. It's important to choose the right transfection method to ensure the best results for your particular experiment.
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