When you are doing qPCR you are quantitating a specific gene so you need to be specific, second during reverse transcription you are trying to transcribe RNA into cDNA so you dont miss any mRNA or miRNA as they can be very small so you just need a primer to anneal to any RNA in your sample to trancribe it, it should not be specific.

When you are doing qPCR you are quantitating a specific gene so you need to be specific, second during reverse transcription you are trying to transcribe RNA into cDNA so you dont miss any mRNA or miRNA as they can be very small so you just need a primer to anneal to any RNA in your sample to trancribe it, it should not be specific.

Random primers are oligodeoxyribonucleotides(hexamers) used to prepare labelled DNA probes from template for filter hybridization and to prime mRNA for cDNA synthesis with and without poly(A).For qPCR primers are specific for gene of interest.

Random primers are used for cDNA synthesis from whole cell RNA (Reverse transcription) while qPCR primer is used for the quantification of gene of interest.

Hello

Many researchers use an OligodT primer so they can get full length copies of the mRNA. However, if the message is long (>4kb) or does not have a poly A tail (prokaryotic mRNA) you can use the random primers. Random primers will enable you to transcribe 5′ ends of long genes, but your cDNAs may not be full length copies of the entire gene. The third choice is a gene specific primer. Gene Specific primers enhance sensitivity by directing all of the RT activity to a specific message instead of transcribing everything in the mix. If you are performing a one-step RT-PCR, gene specific primers are used because the RT primer is also your reverse primer for the PCR step.

References :

# https://worldwide.promega.com/resources/pubhub/faq-for-rt-qpcr/

# https://www.thermofisher.com/hk/en/home/brands/thermo-scientific/molecular-biology/molecular-biology-learning-center/molecular-biology-resource-library/basic-principles-rt-qpcr.html

#https://bitesizebio.com/1810/six-important-factors-for-successful-reverse-transcription/

Good Luck

I agree with all a answers

qPCR primer is used for specific gene of interest.