Can I calculate the average from the data which are collected every 30 minutes for ANOVA?

I artifically elevated the air temperature in the heated plot by 3-celsius degree compared to that in the control for 7 days. I want to figure out the effect of the temperature manipulation on...

21 January 2021 1,185 8 View

What is wrong with my western blot transfer system? (smearing is occured)?

I'm now doing western blotting for several proteins. but transfer is not perfectly happened. bands are looks like smeared or smudged. And it seems to the lower MW bands are more affected. what did...

06 December 2020 9,153 5 View

How to calculate bulk density, porosity, and void ratio of soil?

If the particle diameter is 2 mm and the particle density is 2.5 g/cm, what is the bulk density, porosity, and void ratio of this soil? I had known that the formula to calculate them. The...

11 October 2020 3,466 3 View

How can I check the deparaffinization during IHC protocol?

Hello. I am a beginner researcher experimenting with IHC for the first time. I would like to know how to make sure it is completely deparaffinized when it is fixed with paraffin and removed with...

22 March 2020 7,795 3 View

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

If the cofactor(NAD) concentration in 1mM and substrate is 10mM in enzyme reaction, the substrate become 9mM after reaction?

02 March 2020 3,982 6 View

How to make simulated body fluid using glycerol and ethanol?

Hi. I'm trying to find the method making simulated body fluid using gylcerol and ethanol, but I cannot find it. (I want to make the solution that has similar surface tension and viscosity of human...

05 November 2019 9,051 1 View

How many cells do I need to get a pure plasma membrane? Do you have a kit?

The purpose of this study is to obtain plasma membranes from adherent cells by means of ultracentrifuge. The problem with the experiment is that the yield is too low when obtaining plasma...

01 October 2019 3,375 3 View

Can I use multilevel regression with skewed count data?

I am studying neighbourhood effects on antisocial behaviour using multilevel modelling. The dependent variable, antisocial behaviour is a count data which varies between 0 and 10 which is a skewed...

28 November 2018 8,633 7 View

In sandwich ELISA in human serum, how can I reduce matrix effects and background?

Hello, I'm setting sandwich ELISA to measure amount of target protein in human serum. I have recombinant protein and checked 2 kinds of antibodes(different epitope) were suitable to perform...

29 March 2018 8,728 3 View

Methyl methacrylate polymerization with benzoyl peroxide as initiator... is this harmful?

I am currently working on methyl methacrylate polymerization, with benzoyl peroxide as initiator. Although I always work in the hood, I sometimes end up inhaling a little bit of methyl...

04 October 2017 9,655 4 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How to define the sink temperature and film coefficient?

Hi, everyone. I am considering the heat conviction during compacting simulation by Abaqus. I was wondering that how to define the sink temperature and film coefficient? Is there any references...

21 February 2021 6,197 1 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View