What is the difference between measuring ghrelin in plasma than tissue?

06 June 2018 0 10K Report

I don't know the difference between both or which one is better

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More Ricardo Ramos's questions See All

How to convert 1.06mM to mM/mg?

hi I'd like to know how to convert 1.06mM to mM/mg of sample. I've weighed 100 mg of sample and I diluted in 1 milliliter of water. the molecular weight of the acid (sample) is 116.16g/mol thanks

04 May 2019 3,221 2 View

How to prepare 5 mL of a mixture of MeOH/DMSO/H2O (40:40:20, v/v/v)containing 0.1% HCl. ?

Hi, I have a doubt about this preparation: 5 mL of a mixture of MeOH/DMSO/H2O (40:40:20, v/v/v) containing 0.1% HCl. the 0.1% HCL is taking into account that all the volume (5 mL) is acidified...

04 May 2019 2,024 3 View

Could anyone help me, which is the better pH to preserve ellagic acid?

Hi, could anyone help me please, I was reading an article where it an extraction of ellagic acid of a fruit and they change the pH of ellagic acid to 2.5 but I don´t know why is better to have...

10 November 2018 7,642 3 View

Which statistical test is suitable for comparing concentration of ellagic acid over time?

Hi could someone help me to know what statistical test to use for comparing concentration of ellagic acid over time, I did three measurements of ellagic acid for each time (5hours, 10h, 15h, 20h)...

09 October 2018 4,397 5 View

Can I use both methanol and dmso together to do dpph assay?

Hi could someone help me, I want to do DPPH assay but my samples are in methanol and dmso and I don't know if the samples and stock solution of dpph should be prepared in the same solvent because...

08 September 2018 1,155 2 View

How can I do TLC if I a have my sample with DMSO and they have a pH of 2.5?

please could someone help me? I'm having problems to run my TLC and I think the problem is the dmso that I have in my sample, I have also a pH of 2.5 in it and I don´t know if the pH affects...

08 September 2018 8,529 3 View

Sorry someone knows how many times do I have to measure glucose, triglycerides and cholesterol in mice to have good results?

thanks for your advice

06 July 2018 3,661 2 View

Someone knows a protocol to extract MUC2 of mice to make western blot?

I need to extract munc2 of mice (c57bl/6j) and I'm looking for a method to extract them, thanks

05 June 2018 4,006 1 View

How to know the appropiate age of a mouse for an experiment?

I want to use mice with normal weight

05 June 2018 1,423 2 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 589 4 View

For the process of preparing decellularized matrices what is the ideal solution in which we can bring the tissue from slaughter house to lab?

I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...

31 July 2024 5,645 2 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

How to select relevant metabolites from multiple annotations ?

I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...

24 July 2024 5,789 0 View