How to convert 1.06mM to mM/mg?

hi I'd like to know how to convert 1.06mM to mM/mg of sample. I've weighed 100 mg of sample and I diluted in 1 milliliter of water. the molecular weight of the acid (sample) is 116.16g/mol thanks

04 May 2019 3,132 2 View

How to prepare 5 mL of a mixture of MeOH/DMSO/H2O (40:40:20, v/v/v)containing 0.1% HCl. ?

Hi, I have a doubt about this preparation: 5 mL of a mixture of MeOH/DMSO/H2O (40:40:20, v/v/v) containing 0.1% HCl. the 0.1% HCL is taking into account that all the volume (5 mL) is acidified...

04 May 2019 1,953 3 View

Could anyone help me, which is the better pH to preserve ellagic acid?

10 November 2018 7,585 3 View

Which statistical test is suitable for comparing concentration of ellagic acid over time?

Hi could someone help me to know what statistical test to use for comparing concentration of ellagic acid over time, I did three measurements of ellagic acid for each time (5hours, 10h, 15h, 20h)...

09 October 2018 4,346 5 View

Can I use both methanol and dmso together to do dpph assay?

Hi could someone help me, I want to do DPPH assay but my samples are in methanol and dmso and I don't know if the samples and stock solution of dpph should be prepared in the same solvent because...

08 September 2018 1,104 2 View

How can I do TLC if I a have my sample with DMSO and they have a pH of 2.5?

08 September 2018 8,429 3 View

Sorry someone knows how many times do I have to measure glucose, triglycerides and cholesterol in mice to have good results?

thanks for your advice

06 July 2018 3,602 2 View

Someone knows a protocol to extract MUC2 of mice to make western blot?

I need to extract munc2 of mice (c57bl/6j) and I'm looking for a method to extract them, thanks

05 June 2018 3,964 1 View

How to know the appropiate age of a mouse for an experiment?

I want to use mice with normal weight

05 June 2018 1,364 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

IF troubleshooting: looking at an intracellular protein that localizes to the cell's and how to reduce increase in signal due to cell ruffles?

I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...

24 February 2021 8,582 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View