I need to extract munc2 of mice (c57bl/6j) and I'm looking for a method to extract them, thanks
Just see if the attached article would help.
hi I'd like to know how to convert 1.06mM to mM/mg of sample. I've weighed 100 mg of sample and I diluted in 1 milliliter of water. the molecular weight of the acid (sample) is 116.16g/mol thanks
04 May 2019 3,221 2 View
Hi, I have a doubt about this preparation: 5 mL of a mixture of MeOH/DMSO/H2O (40:40:20, v/v/v) containing 0.1% HCl. the 0.1% HCL is taking into account that all the volume (5 mL) is acidified...
04 May 2019 2,024 3 View
Hi, could anyone help me please, I was reading an article where it an extraction of ellagic acid of a fruit and they change the pH of ellagic acid to 2.5 but I don´t know why is better to have...
10 November 2018 7,642 3 View
Hi could someone help me to know what statistical test to use for comparing concentration of ellagic acid over time, I did three measurements of ellagic acid for each time (5hours, 10h, 15h, 20h)...
09 October 2018 4,397 5 View
Hi could someone help me, I want to do DPPH assay but my samples are in methanol and dmso and I don't know if the samples and stock solution of dpph should be prepared in the same solvent because...
08 September 2018 1,155 2 View
please could someone help me? I'm having problems to run my TLC and I think the problem is the dmso that I have in my sample, I have also a pH of 2.5 in it and I don´t know if the pH affects...
08 September 2018 8,529 3 View
thanks for your advice
06 July 2018 3,661 2 View
I don't know the difference between both or which one is better
05 June 2018 10,037 0 View
I want to use mice with normal weight
05 June 2018 1,423 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View