How can I do TLC if I a have my sample with DMSO and they have a pH of 2.5?

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Hamidreza Rezania

Dear Ricardo ,

you can not perform TLC with DMSO, except first spot on tlc plate, dry its solvent with hair dryer then do the TLC test.

Sharad Medhe

DMSO samples not recommanded in TLC. Remove the DMSO and add volatile solvent like Methanol or chloroform in sample and run it for TLC

Ricardo Ramos

thanks for your advice, actually I have my samples in methanol:dmso (1:1) cause I used them in hplc and now I wanna do TLC