How to convert 1.06mM to mM/mg?

hi I'd like to know how to convert 1.06mM to mM/mg of sample. I've weighed 100 mg of sample and I diluted in 1 milliliter of water. the molecular weight of the acid (sample) is 116.16g/mol thanks

04 May 2019 3,132 2 View

How to prepare 5 mL of a mixture of MeOH/DMSO/H2O (40:40:20, v/v/v)containing 0.1% HCl. ?

Hi, I have a doubt about this preparation: 5 mL of a mixture of MeOH/DMSO/H2O (40:40:20, v/v/v) containing 0.1% HCl. the 0.1% HCL is taking into account that all the volume (5 mL) is acidified...

04 May 2019 1,953 3 View

Could anyone help me, which is the better pH to preserve ellagic acid?

10 November 2018 7,585 3 View

Which statistical test is suitable for comparing concentration of ellagic acid over time?

Hi could someone help me to know what statistical test to use for comparing concentration of ellagic acid over time, I did three measurements of ellagic acid for each time (5hours, 10h, 15h, 20h)...

09 October 2018 4,346 5 View

Can I use both methanol and dmso together to do dpph assay?

Hi could someone help me, I want to do DPPH assay but my samples are in methanol and dmso and I don't know if the samples and stock solution of dpph should be prepared in the same solvent because...

08 September 2018 1,104 2 View

How can I do TLC if I a have my sample with DMSO and they have a pH of 2.5?

08 September 2018 8,429 3 View

Someone knows a protocol to extract MUC2 of mice to make western blot?

I need to extract munc2 of mice (c57bl/6j) and I'm looking for a method to extract them, thanks

05 June 2018 3,964 1 View

What is the difference between measuring ghrelin in plasma than tissue?

I don't know the difference between both or which one is better

05 June 2018 9,973 0 View

How to know the appropiate age of a mouse for an experiment?

I want to use mice with normal weight

05 June 2018 1,364 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View