But i have loading efficiency more important that loading capacity; is it normal ?
Here you may find an answer:
https://www.researchgate.net/post/How_do_I_calculate_encapsulation_efficiency/1
https://www.researchgate.net/publication/24431519_Novel_self-assembled_core-shell_nanoparticles_based_on_crystalline_amorphous_moieties_of_aliphatic_copolyesters_for_efficient_controlled_drug_release
Article Novel self-assembled core-shell nanoparticles based on cryst...
About the following formulation,
%LC = [Entrapped Drug/nanoparticles weight] * 100,
should it be corrected to
Entrapped Drug/(nanoparticles + entrapped drug) weight*100
?????
This is a tricky question. In my view of point it depends on your formulation.
Entrapment Efficiency is defined as the amount of drug in the total amount of microparticles collected at the end of the manufacturing process divided by the theoretical expected amount of drug in that total amount of microparticles ( which means total solid recovered, e.g. polymer wt. + drug wt.)
Non-entrapped is a tricky part! How could you wash (measure) the non-entrapped without underestimation your value. There will be always some particles leaked during the measurement.
The very simple way to measure the entrapment efficiency is first, weigh out the whole batch, this is called the recovered MPs. Second, take a known amount of this batch (e.g. 30 mg) and find out how much of your drug is in there. Do the proportion calculation to find out how much would you expect in the whole batch. This is called the actual (expected) amount of drug in the recovered MPs. Finally,
% Encapsulation Efficiency = actual amount of drug in recovered MPs/ Theoretical amount of drug in total solid recovered MPs.
The reason we went with the recovered instead of the originally fed solution because we are not sure how much the lost of the drug in MPs might be.
That lost occurred during the manufacturing and we have no idea how much might have from our drug. However, the recovered MPs, we do have them and we measured the EE from them in triplicate. That will give us a stronger evidence regarding the claim about the EE of the batch.
You don’t need to know what is loading capacity! The previous knowledge at the top is enough to know how much of your microparticles expected to have the drug!
Loading efficiency gives you an idea about the %drug that is successfully entrapped/adsorbed into nanoparticles. It is calculated as follows:
%LE = [(Drug added - Free "unentrapped drug")/Drug added] *100
Example: If the %LE is 30%, it means that 30% of your drug is entrapped into the nanoparticles.
Loading capacity helps you to deal with nanoparticles after their separation from the medium and to know their drug content. It is calculated using the following equation:
%LC = [Entrapped Drug/nanoparticles weight] * 100
Dear all Hi,
How to calculate the Free "unentrapped drug" ? in aforementioned calculations?
The unentrapped can be calculated by washing the microcapsules or nanoparticles, centrifuge them, and calculate the amount of the drug (or e.g., protein) in the supernatant using a suitable assay according to your entrapped material, assuming the coat will not dissolve in the solvent.
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