Hello

Antigen retrieval dependent of antibody and the higher pH can lead to background staining.

for clear information see these links

Good Luck

http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3201121/

https://www.researchgate.net/post/How_do_you_choose_the_pH_for_your_antigen_retrieval_solution

http://www.ihcworld.com/epitope_retrieval.htm

http://jhc.sagepub.com/content/53/11/1311.full

http://jhc.sagepub.com/content/43/2/193.full.pdf

Dear Faez Amokrane Nait Mohamed

Pls. find the attached files

https://www.rndsystems.com/resources/protocols/antigen-retrieval-methods

https://www.bio-rad-antibodies.com/antigen-retrieval-techniques-for-use-with-formalin-fixed-paraffin-embedded-sections.html

http://jhc.sagepub.com/content/45/3/327.full

http://www.abcam.com/kits/antigen-retrieval-for-ihc

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3201121/

http://www.ihcworld.com/_technical_tips/antigen_retrieval_tips.htm

http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol

https://www.researchgate.net/profile/Clive_Taylor2/publication/15375984_Shi_SR_Imam_SA_Young_L_et_al_Antigen_retrieval_immunohistochemistry_under_the_influence_of_pH_using_monoclonal_antibodies/links/0c960523788f3d96a4000000.pdf

https://www.researchgate.net/profile/Shan-Rong_Shi/publication/11247725_DNA_Extraction_from_Archival_Formalin-fixed_Paraffin-embedded_Tissue_Sections_Based_on_the_Antigen_Retrieval_Principle_Heating_Under_the_Influence_of_pH/links/546f94b10cf2d67fc03118cd.pdf

Hoping this will be helpful

Regards

Prof. Houda Kawas

Article Antigen retrieval immunohistochemistry under the influence o...

Article DNA Extraction from Archival Formalin-fixed, Paraffin-embedd...

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