immunohistochemestry; IHC; antigen retrieval
Hello
Antigen retrieval dependent of antibody and the higher pH can lead to background staining.
for clear information see these links
Good Luck
http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3201121/
https://www.researchgate.net/post/How_do_you_choose_the_pH_for_your_antigen_retrieval_solution
http://www.ihcworld.com/epitope_retrieval.htm
http://jhc.sagepub.com/content/53/11/1311.full
http://jhc.sagepub.com/content/43/2/193.full.pdf
Dear Faez Amokrane Nait Mohamed
Pls. find the attached files
https://www.rndsystems.com/resources/protocols/antigen-retrieval-methods
https://www.bio-rad-antibodies.com/antigen-retrieval-techniques-for-use-with-formalin-fixed-paraffin-embedded-sections.html
http://jhc.sagepub.com/content/45/3/327.full
http://www.abcam.com/kits/antigen-retrieval-for-ihc
http://www.ihcworld.com/_technical_tips/antigen_retrieval_tips.htm
https://www.researchgate.net/profile/Clive_Taylor2/publication/15375984_Shi_SR_Imam_SA_Young_L_et_al_Antigen_retrieval_immunohistochemistry_under_the_influence_of_pH_using_monoclonal_antibodies/links/0c960523788f3d96a4000000.pdf
https://www.researchgate.net/profile/Shan-Rong_Shi/publication/11247725_DNA_Extraction_from_Archival_Formalin-fixed_Paraffin-embedded_Tissue_Sections_Based_on_the_Antigen_Retrieval_Principle_Heating_Under_the_Influence_of_pH/links/546f94b10cf2d67fc03118cd.pdf
Hoping this will be helpful
Regards
Prof. Houda Kawas
Article Antigen retrieval immunohistochemistry under the influence o...
Article DNA Extraction from Archival Formalin-fixed, Paraffin-embedd...
Also
For surface nanoparticles functionalization, Thanks
11 December 2017 600 6 View
10 November 2016 2,749 6 View
Hi everybody, can someone help me with some french books (springer, reviews, thesis) talking about nanoparticles vaccins, especially polymeric nanoparticles adjuvants?Thanks a lot.
04 May 2016 6,778 0 View
Thank you so much
03 April 2016 8,258 11 View
What's the difference between accelerating voltage of 80 kV ans 50 kV on the TEM analysis of polymeric nanoparticles ?Thanks all.
01 February 2016 3,487 3 View
08 September 2015 3,969 0 View
Drug delivery system, Vaccine adjuvant
07 August 2015 3,183 0 View
Can we evaluate-it on animals, or only in humans?
02 March 2015 3,978 8 View
It's supposed to be a great news, how information comes to you?
31 December 2014 7,704 9 View
Of nanoparticle or particle encapsulation. Thanks
11 December 2014 6,417 9 View
I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why
02 March 2021 1,388 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...
01 March 2021 6,999 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.
24 February 2021 393 14 View
Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...
24 February 2021 1,243 1 View
I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...
23 February 2021 7,842 1 View
I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...
23 February 2021 9,933 1 View