How can I prepare a MES buffer pH=6 ?

For surface nanoparticles functionalization, Thanks

11 December 2017 600 6 View

Why do we use pH 6 or pH 9 for antigen retrieval in immunohistochemestry (IHC)?

immunohistochemestry; IHC; antigen retrieval

10 November 2016 7,441 3 View

French books talking about nanoparticles vaccins?

Hi everybody, can someone help me with some french books (springer, reviews, thesis) talking about nanoparticles vaccins, especially polymeric nanoparticles adjuvants?Thanks a lot.

04 May 2016 6,778 0 View

What is the difference between XRD and X-ray fluorescence (XRF) in nanoparticles characterization?

Thank you so much

03 April 2016 8,258 11 View

What's the difference between accelerating voltage of 80 kV ans 50 kV on the TEM analysis of polymeric nanoparticles ?

What's the difference between accelerating voltage of 80 kV ans 50 kV on the TEM analysis of polymeric nanoparticles ?Thanks all.

01 February 2016 3,487 3 View

Has anyone some pictures showing the "swelling" of nanoparticles encapsulating antigens?

08 September 2015 3,969 0 View

Does anyone work on calcium-alginate nanotechnology ?

Drug delivery system, Vaccine adjuvant

07 August 2015 3,183 0 View

Can someone explain me what's the difference between reactogenicity and the immunogenicity of a vaccine?

Can we evaluate-it on animals, or only in humans?

02 March 2015 3,978 8 View

How did you know that a member of your team has published a new scientific paper ?

It's supposed to be a great news, how information comes to you?

31 December 2014 7,704 9 View

What is the difference between loading capacity and loading efficiency?

Of nanoparticle or particle encapsulation. Thanks

11 December 2014 6,417 9 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View