When seen under microscope how to differentiate between cell clumps and tumerspheres. How to count them?
One would start will few single cells (filtered etc.) to avoid clumping in the culture, then you increase the confidence level of your assay that you obtain tumorospheres.
I want to dock a secretory protein having about 110 amino acid residues with more than thousand receptor proteins to find the best interaction. Is there any highthroughput technique available to...
06 January 2023 1,420 2 View
The conventional protocols for miRNA hybridization for FFPE tissue sections stretch for 2-3 days, with prehybridization and an overnight hybridization steps. But some companies like thermo and...
21 June 2022 6,199 0 View
Step by step procedure
10 January 2022 5,740 3 View
What is the purpose of education and what we get from education?
23 September 2020 6,097 4 View
I am working on a kinase-inhibitor complex. I wanted to perform a molecular dynamics simulation for the same. While going through the literature, I came across several options for the water model...
04 July 2020 8,106 0 View
I have developed a homology model with different templates. For checking the overall deviation from template structure, RMSD calculation was performed using Match Maker option in Chimera. It gives...
29 June 2020 7,133 0 View
Forgive me for being Naive. I have read that the postprandial concentration normally doesn't go above 140mg/dl, anything above it is considered diabetic. for instance:...
23 July 2019 2,088 4 View
I work on MC3T3-E1 cell lines. The cells appear absolutely fine in flasks, but when I seed them in 12/24 well plates for mineralization (nunc thermofischer plates), then cells appear to die in few...
03 February 2019 1,772 3 View
During animal cell culture, confluent cells are trypsinised, centrifuged, resuspended and finally subcultured. I read somewhere that contact inhibition is used to make the cultures synchronous....
03 February 2019 561 3 View
I have lot of CV (text documents). From these data I want to extract particular section of 'Education Qualification', 'Experience', etc.
03 January 2019 4,590 12 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...
30 July 2024 2,349 1 View
Hi, In a few wells and cells, we’ve noticed an unusual signal in the nucleus using both eppifluroscence & confocal microscopes. Does anyone know why this is happening? It occurred twice in...
10 July 2024 1,870 0 View
how can I incorporate the dyes to each phase ? do I have to heat them !!
21 June 2024 9,377 1 View
fungal inoculum treated with lacto phenol blue and observed under microscope anyone help me to identify and share some information regarding this fungal slide
07 June 2024 9,647 3 View
I have been trying to image a receptor in the brain for weeks now. It is an EGFP-cre G-coupled protein receptor. The model is a iCre based EGFP KI mouse model crossed with an Ai9 reporter line. I...
23 May 2024 7,776 2 View
A Comprehensive Overview of Continuous Renal Replacement The... How does CRRT differ from intermittent hemodialysis and peritoneal dialysis in terms of treatment duration and methodology?
12 May 2024 6,912 0 View
This is a transmission electron microscope picture of salivary gland secretory cells. What is the structure in the picture?
08 May 2024 1,397 4 View
I need to determine the area of the ossified regions and the ossification percentage, but to do that accurately, I first need to measure the total bone area. I'm using Fiji software for this task,...
03 May 2024 1,034 1 View