How much is the role of education in living a happy life?

What is the purpose of education and what we get from education?

23 September 2020 5,949 4 View

What should be the standard glucose concentration to be used in high glucose studies, that match the physiological levels?

Forgive me for being Naive. I have read that the postprandial concentration normally doesn't go above 140mg/dl, anything above it is considered diabetic. for instance:...

23 July 2019 1,969 4 View

What could be the possible reasons behind the cells dying in the culture plate in few wells while surviving in other, media conditions being same?

I work on MC3T3-E1 cell lines. The cells appear absolutely fine in flasks, but when I seed them in 12/24 well plates for mineralization (nunc thermofischer plates), then cells appear to die in few...

03 February 2019 1,648 3 View

How to extract particular section from text data using NLP in Python?

I have lot of CV (text documents). From these data I want to extract particular section of 'Education Qualification', 'Experience', etc.

03 January 2019 4,454 12 View

Is Nested PCR approach to detect HPV infection in fresh as well as FFPE specimens right?

Hi! I am using nested PCR approach to detect mucosal HPV in cervical specimens as well as from FFPE tissues. This is to increase the sensitivity. But in doing so, I am getting a same similar size...

28 March 2018 9,631 4 View

How to prepare drug library?

There is no known inhibitor for my protein, so i want to screen some drugs which are natural compounds and most probably commercially available, so for the drug library, which database i used to...

03 October 2017 505 2 View

Pyruvate kinase M2 assay?

We have synthesized some inhibitors for PKM2 enzyme. we are planning to perform enzyme kinetic studies. we studied some protocols for the same but conc. of enzymes to be used are missing. some of...

18 September 2017 433 4 View

PLKO1 vectors transduction?

We are going to use SHC001 Scramble vector from sigma it does not contain any shRNA or GFP tag, however, it has ampicillin and puromycin resistance genes. In this case, how to characterize that...

12 September 2017 7,703 6 View

Which form of sh RNA is best for experimental purposes?

20 July 2017 4,414 3 View

Cancer stem cell isolation protocol?

Hello, I am isolation cancer stem cells from MCF-7 cells. I saw protocols for 96 well plate where 1 cell per well is seeded. How many cells should be seeded in 6 well plate.

29 March 2017 5,174 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Troubleshooting with immunofluoresence/confocal microscope? What went wrong?

Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...

11 February 2021 562 6 View

In your view, which current SEM is most recommended for EBSD/TKD and EDS analysis?

I know that this is a very difficult question, as the areas of application can vary greatly. However, if you want to operate low as well as high resolution (from a few millimeters to nanometers),...

02 February 2021 4,985 4 View

What can cause laser beam polarisation fluctuations?

Dear colleagues, We have observed large fluctuations in laser power in our setup and traced them down to fluctuations in laser beam polarisation. Attached is a schematic of the relevant part of...

14 January 2021 7,505 4 View

Data misrepresentation and Ignorance of socio-economic factors?

Do you think the majority of developing, underdeveloped, and even the developed countries lack in capturing the finer elements of behavioral data generated at various interactive and reactive...

13 January 2021 3,241 2 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View

Are these fungi or is it just medium impurity?

I just thawed my 4T1 cancer cells and 24 hours after seeding the cells in the T25 flask there were somethings beside my cells. The culture medium is pretty clear and there is no more than 6 or 7...

28 December 2020 5,928 3 View

How to prediction non-visible parts of microscopic pictures?

Dear all, I like to ask if someone has experience with evaluating pictures towards the prediction of non-visible parts. I have confocal-laser-scanning microscopic pictures of yeast cells and want...

28 December 2020 4,309 11 View

The analysis of the following microscop observation?

I use the BM medium, which was usually for amoeba growth, to cultivate the microorganism in water. The picture is observed by microscope after gram-staining. Can someone please tell why the image...

20 December 2020 9,719 3 View