I will be carrying out some biofilm removal and biofilm prevention assay experiments soon but am confused what this actually means.
In biofilm removal assays, is the compound added at the same time the bacteria is added to the wells?
In biofilm prevention assays, is the bacteria added to the wells, incubated for 24h and then the compound is added and incubated for a further 24h?
Hi Chau,
I think your description of the 2 assays should be reverse, which means:
- Biofilm inhibition assay ("biofilm prevention" in your words): the compound and bacteria are added at the same day
- Biofilm eradication assay ("biofilm removal" in your words): the bacteria are cultured in wells for 24h and then compound will be added and incubated for another 24h
You could refer to this article for more information (if you have the accession): https://www.nature.com/articles/s41596-021-00515-3?proof=tr
Hi Chau
Prevention assay would assess the ability of the agent/ process etc in respect that the biofilm is not allowed to form. Where as biofilm removal assay focuses exclusively on the ability of the agent/process etc to remove and disperse the biofilm.
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