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Questions related from Michael Chau
I have been growing E. coli and P. aeruginosa strains in my biofilm formation assays. However recently, after washing the crystal violet off with water, there appears not to be any rings of...
11 September 2022 9,724 0 View
I'm currently carrying out biofilm assays with E.coli and P.aeruginosa in microtiter plates and was going to use the following as a positive control in my experiment: 100ul bacteria + 100ul...
04 February 2022 5,785 2 View
I made up three separate solutions of M9 minimal media adding the components in the following order (total volume 200ml) and then adjusted the pH to 7, 7.4 and 7.8 1. 40ml M9 salts (5x) 2.400ul...
02 December 2021 2,617 0 View
I will be carrying out some biofilm removal and biofilm prevention assay experiments soon but am confused what this actually means. In biofilm removal assays, is the compound added at the same...
13 November 2021 7,495 2 View
I have measured in a cuvette my E.coli culture diluted down to OD of 0.01 and also pipetted the solution into a microtiter plate and read at OD595 (also read in spectrophotometer) but my...
13 November 2021 341 1 View
In my experiments using E.coli K-12, the planktonic and biofilm absorbance readings taken at OD600 during the biofilm formation assay are all below 0.1. I have some suggestions of possible sources...
06 August 2021 6,093 1 View
