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I have been growing E. coli and P. aeruginosa strains in my biofilm formation assays. However recently, after washing the crystal violet off with water, there appears not to be any rings of...
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I'm currently carrying out biofilm assays with E.coli and P.aeruginosa in microtiter plates and was going to use the following as a positive control in my experiment: 100ul bacteria + 100ul...
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I made up three separate solutions of M9 minimal media adding the components in the following order (total volume 200ml) and then adjusted the pH to 7, 7.4 and 7.8 1. 40ml M9 salts (5x) 2.400ul...
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I will be carrying out some biofilm removal and biofilm prevention assay experiments soon but am confused what this actually means. In biofilm removal assays, is the compound added at the same...
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