What is the definition of begin and end of a fed-batch cycle operation in Microbial fuel cells (MFCs)/Microbial electrolysis cells(MECs)?

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Does anybody know how to calculate COD removal in fed-batch Microbial electrolysis cell (MEC)?

calculate COD removal in fed-batch Microbial electrolysis cells (MECs)

08 September 2014 9,610 3 View

How can we determine duration of one fed-batch cycle in Microbial fuel cells (MFCs)/Microbial electrolysis cells(MECs)?

determine duration of one fed-batch cycle in MFC/MEC

08 September 2014 7,346 1 View

Dose anybody know how to separate CO2, CO and H2 gases from air (atmosphere)?

Separate CO2, CO and H2 gases from air (atmosphere).

09 August 2014 4,721 2 View

Does anybody know of a list of Electrochemically Active Bacteria (EAB) which were used in Microbial electrolysis cell (MEC)/(MFC) studies so far?

The two most-studied Electrochemically active bacteria (EAB) are Geobacter sulfurreducens PCA and Shewanella oneidensis MR-1.

08 August 2014 625 2 View

Dose anybody know what are the differences between operated in fed-batch mode/operated in batch mode/operated under continuous flow MFC/MEC?

I want to know the definition of operated in fed-batch mode/operated in batch mode/operated under continuous flow Microbial Fuel Cell (MFC) / Microbial Electrolysis Cell(MEC).

06 August 2014 8,670 5 View

Dose anybody know how to measure anode potential in Microbial electrolysis cell (MEC)?

Anode potential in Microbial electrolysis cell (MEC)

06 August 2014 406 4 View

Does anybody know how can I maintain anaerobic condition in a Microbial Electrolysis Cell (MEC)?

I am working on MEC research and I need to maintain anaerobic condition in MEC reactor.

30 July 2014 1,604 12 View

How to measure the conductivity of Microbial electrolysis cell (MEC)?

Measure the conductivity of MEC.

29 July 2014 2,253 0 View

Can anyone suggest how to enhance hydrogen production in the microbial electrolysis cells?

Hydrogen production in single chamber microbial electrolysis cell.

28 July 2014 7,681 7 View

Can anyone give information about controlling the pH in Microbial Electrolysis Cell (MEC)?

I am working on Microbial Electrolysis Cell (MEC). I would like to know how I can control the pH in MEC test.

27 July 2014 3,753 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

What are the best (less toxic to human) methods of microbial lab fumigation?

We are planning to establish a microbial laboratory in our research center and are actively seeking the best disinfection methods. If anyone has any recommendations, please let us know. Thank you.

04 August 2024 1,973 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Bin Lai

You an use current or potential as the standard reference. set a value X, while I or V > X, it is the start point, and while I or V < X, it reaches the end. It is good to have the rule for the end point, and you can take the start point as where you inoculate your reactors.

Dipak A Jadhav

During batch cycle, when you feed the reactor with influent is the begin of cycle. At the end, when u removed effluent is the end of cycle. Its depends upon how fast microbes utilizing that feed.