What is the current protocol(s) for sampling for molecular characterization of marine fungi from live substrates (cnidarians, crustaceans, etc.)?

16 July 2014 1 2K Report

I wish to identify/characterize marine fungi associated with various marine organisms found on tropical reefs. I have found numerous papers on sampling of sediments and wood fragments but need protocol for live substrate sampling.I would also love to establish a line of communication with a marine mycologist.

Mohammd Abdigoudarzi

The first step is DNA extraction , then universal primers could be selected for fungi identification, according to different manuscript written in the field. then you can narrow your research and go ahead.

Regards

Dr. Abdigoudarzi

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