We have isolated endophytic fungus from lichen and orchid. Now, we want to do genetic characterization.
Being in resource poor setting, we need reliable, cheap manual method of DNA isolation.
Please suggest some protocol. We'll be really grateful to you.
Thank you.
Kind regards,
Bivek
I think the PCR based molecular technique would be more suitable for you. For detailed methodology please consult the manuscript published in Plant Pathology & Quarantine 5(2): 74-81 (2015) on "A rapid and efficient method of fungal genomics suitable for PCR based molecular method" by Aamir et. al.
Hi, simple phenol-chloroform-extraction. Worked fine for fungal DNA isolation (lichen fungi) and is way cheaper than DNA-isolation kits.
Dear Tarafdar and Annette,
Thank you for the relevant information. We will go through the same procedure (phenol-chloroform) extraction.
kind regards,
Bivek
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