Bivek Timalsina

8 Questions 17 Answers 0 Followers

Questions related from Bivek Timalsina

What is the cost effective relevant protocol for Fungal DNA isolation?

We have isolated endophytic fungus from lichen and orchid. Now, we want to do genetic characterization. Being in resource poor setting, we need reliable, cheap manual method of DNA...

19 September 2016 5,295 3 View

What should be used to compare three diagnostic methods for determining their efficiency?

I have to compare among ZN microscopy, Fluorescent microscopy and Culture in their efficacy. Which test methods is more applicable in this regard. Can I use Chi-square test to determine...

04 March 2015 3,870 15 View

What is the proper number of HEK-293T cells to be seeded in six well plate, so that I can maintain the cells for 48 hours?

I am using HEK-293T cells for transfection with plasmid DNA of pNL4-3 and p-INDIEC-1. I am getting good expression of pNL4-3 using Lipofectamine 2000 and cells are also in good condition after...

22 February 2014 9,964 5 View

Which competent cell is suitable for transformation of pIndie-C1 DNA and what are the transformation criteria?

I have done transformation of pIndie-C1 DNA (using 100 uL ; 0.6 ug/uL) in Top 10 competent cells using heat shock method. 400 uL was transferred to 250 uL LB broth and 100 uL was plated in LB...

10 January 2014 4,272 3 View

Why does RNA Polymerases of (-) strand viruses copy viral RNAs only when they are present in the nucleocapsid?

VSV genomic RNA is a template for RNA polymerase only when it is bound to the nucleocapsid protein N.

10 September 2013 356 1 View

Can I thaw HEK-293 cell line in ice?

I have done thawing in water bath with gentle shaking for 1-2 minute, and tried to do thawing in ice as well, but the result was not satisfactory. What could be the reason for it? I would also...

07 September 2013 423 1 View

What will happen to Huh-7 cell line if we use 5% CO2 for DMEM medium rather than 10% CO2?

We have to optimize the growth characteristic for cell line culture. Ph is a very important factor for this. We want to use both RPMI and DMEM medium in the same CO2 incubator. But the CO2...

22 August 2013 3,410 3 View

Why Chimpanzees are not infected with Malaria Parasite, Plasmodium falciparum though Human and Chimpanzee are more closely related?

It is found that, when the blood from Human infected with Plasmodium falciparum is injected into the Chimpanzee, they don't show any severe effect as in Human and similarly when the blood from...

10 April 2013 1,292 6 View