Hi everybody,
I am working with MKN45 cell line that needs RPMI medium supplemented with 10% FBS for proliferation in cell culture flask. For viability assay using MTT cultured in 96-well plate, is it logical to use lower FBS concentration like 3% to mimic in vivo conditions? Moreover, 10% FBS may neutralize our drug so, using lower FBS concentration decreases this effect.
Dear Arash Azimzadeh-Isfanjani it is possible to lower concentration of FBS in some cancer cell lines but you should check MKN45 cell requirement and or in possible you can gradually adapt your cells to lower concentration of FBS in which reduce FBS level to 7%, then 5%, and so on. unfortunately I don't know to what extend MKN45 cell would tolerate FBS reduction.
Thank you Kebria. I do not necesserily want to lower the FBS. For proliferation, MKN45 cell line needs 10% FBS in medium. I just mean is it logical to lower the FBS concentration or continue with 10% FBS for viability assay.
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