6 Questions 4 Answers 0 Followers
Questions related from Arash Azimzadeh-Isfanjani
Hi everybody, I am working with MKN45 cell line that needs RPMI medium supplemented with 10% FBS for proliferation in cell culture flask. For viability assay using MTT cultured in 96-well plate,...
01 December 2024 9,782 2 View
Please contribute to this question with your assured knowledge. In treating cells for different time spans like 24h, 48h and 72h, should we seed different numbers of the cells suitable for...
05 December 2021 1,890 4 View
Dear researchers; A question arose for me about Real-Time analysis. I would appreciate you to comment about the question. For expression analysis we need a housekeeping gene to normalize Ct...
28 January 2019 8,678 3 View
Dear friends, The picture in the link below shows Melt Curve for the primer I have for the miRNA I am working with. This is the best set up that can accur for all 40 cDNAs. I myself think it is...
13 January 2014 3,252 6 View
This is the best set up for the primer I have for the miRNA I am working with. It is same for all 40 samples. I myself think it is not applicable but, I need your comments. Thank you all.
12 January 2014 5,678 7 View
I'm doing my my thesis on miRNA differential expression. A question arose when preparing for standard curve, should cDNA or its PCR product be used? When can we use each of them? Another question...
15 June 2013 6,871 3 View
